Quantifying key variables of damage to wheat and barley by Syringopais temperatella (Lepidoptera: Scythrididae)
Cuantificación de variables clave en los daños al trigo y cebada por Syringopais temperatella (Lepidoptera: Scythrididae)
Ihab H. GhabeishI; Firas A. Al-zyoudII; Dhia S. HassawiIII
IDr. Ihab H. Ghabeish, Associate Prof. of Biological Control, Dept. of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan, Fax 00962 53555735, Tel. (mobile): 00962 795222945. balappuniv@yahoo.com. Corresponding Author ]]>
IIDr. Firas A. Al-Zyoud, Associate Prof. of Biological Control and IPM, Department of Plant Protection and IPM, Faculty of Agriculture, Mu'tah University, Karak, 61710 Jordan, P.O. Box: 7, Tel. (mobile): 00962-(0)-797441900. firaszud@yahoo.com
IIIProf. Dr. Dhia Hassawi, Dept. of Biology, Faculty of Science, Al-Anbar University, Al-Anbar, Iraq. dhassawi@yahoo.com
ABSTRACT
Wheat and barley are the principal food crops for millions of people in the predominantly mixed crop-livestock farming systems. The cereal leafminer, Syringopais temperatella (Lepidoptera, Scythrididae) is a major biotic constraint to wheat and barley production in Jordan and the region. Three variables of yield components were used to quantify the loss of wheat and barley including the grain yield, straw dry mass and leaf area consumed. Forty-two (in the field experiment) and one hundred eighteen (in the laboratory experiment) accessions of wheat and barley were used to access the average damage; representing different levels of susceptibility to S. temperatella. Equations of three negative correlations of percentage of infestation versus both grain yield and straw dry mass, and the number of larvae versus the straw dry mass were produced. Results indicated that the average grain yield reduction for both crops due to pest infestation was estimated at 36.1% for wheat and 50% for barley, but the individual reductions ranged 17.4-73.3%. The straw dry mass reduction was estimated at 29.9% for wheat and 16.5% for barley with individual reductions ranging between 8.4 and 44.8%. In addition, one positive correlation for the number of leafminer larvae versus the leaf area consumed was found. The estimated leaf area consumed per one larva was 0.30 cm2 and 0.38 cm2 for wheat and barley, respectively.
Key words: Cereal leafminer; Grain and straw damage; Leaf area consumed.
RESUMEN
El trigo y la cebada son los principales cultivos alimenticios para millones de personas en los sistemas de producción de cultivos y ganadería predominantemente mixtas. El minador de cereales, Syringopais temperatella (Lepidoptera: Scythrididae) es una importante limitación biótica de producción de trigo y cebada en Jordania y la región. Tres parámetros de daño fueron utilizados para cuantificar la pérdida en trigo y cebada: el rendimiento de grano, masa seca de paja y área foliar consumida. Cuarenta y dos (en experimentos de campo) y ciento dieciocho (en experimentos de laboratorio) accesiones de trigo y cebada se utilizaron para estimar el daño promedio, que representan diferentes niveles de susceptibilidad a S. temperatella. Se construyeron las ecuaciones de tres correlaciones negativas para porcentaje de infestación en comparación con el rendimiento de grano y materias seca de paja, y la del número de larvas en comparación con la masa seca de paja. Los resultados indicaron una reducción promedio de rendimiento de grano para los dos cultivos debido a la infestación de plaga estimada en 36,1% para el trigo y 50% para la cebada, pero las reducciones individuales oscilaron entre 17,4-73,3%. La reducción de la masa seca de paja se estimó en 29,9% para el trigo y 16,5% para la cebada con reducciones individuales que variaron entre 8,4 y 44,8%. Además, se encontró un aumento, con correlación positiva, en el número de larvas de minador frente a la zona consumida de hoja. El área foliar estimada consumida por una larva es de 0,30 cm2 y 0,38 cm2 para trigo y cebada, respectivamente.
Palabras clave: Minador de cereales; Daños grano y paja; Área foliar consumida.
Introduction
Wheat and barley are the most planted cereal crops in Jordan with cultivated area of 27,856 and 41,264 hectares in 2011, respectively. However, Jordan is not self-sufficient in these crop productions and depends on imports to cover the national needs. In 2010, Jordan produced 26,659 and 26,711 tons and imported 1,076,000 and 447,000 tons of wheat and barley, respectively (Jordan Statistical Yearbook 2011). Both crops are classified as low-input crops to farmers; hence they cannot accept yield loss due to pest attack. One of the most important insect pests on wheat and barley in Jordan is the cereal leafminer, Syringopais temperatella Lederer (Lepidoptera, Scythrididae). In the Middle East, S. temperatella is a major pest of wheat and barley, and it causes quantitative and qualitative damages through feeding on plant leaf tissues (Jemsi et al. 2002; Jemsi and Rajabi 2003; Gozuüacik et al. 2008; Al-Zyoud 2012, 2013). Duran et al. (1979) found that the insect damage in Turkey reached 22%, while Kaya (1976) estimated the damage to be around 40-60%. In Iraq, crop losses caused by the pest on wheat and barley ranged between 10 and 20% (Abu-Yaman 1971). In Jordan, the pest was reported since more than 50 years ago (Klapperich 1968). It was considered as a non-serious pest on wheat and barley, but since 2001 the insect has become a destructive pest in the south of the country, especially in Karak District (Al-Zyoud 2008; Al-Zyoud et al. 2011) with infestation reaching up to 70% (Al-Zyoud 2013).
Little is known about the association of S. temperatella infestation to the grain and straw yield of wheat and barley. Al-Zyoud (2012) provided results on the leaf area consumed by the cereal leafminer larvae for only six local cultivars of wheat and barley in an attempt to sort out the preferred ones. Unfortunately, his study did not provide information on the number of larvae that consumed that leaf areas.
Pest management is an intensive decision making system requiring efficient decision tools. Decisions require information on crop losses due to pest for control intervention and research prioritization. Traditionally, the crop losses have been determined through empirical yield-infestation relations. Therefore, the current study is undertaken to provide formulas for predicting the grain yield, straw dry mass and plant-leaf area consumed of wheat and barley crops due to the cereal leafminer at a certain infestation level and number of larval attack.
Materials and methods
Field experiment. Seeds of twenty-seven wheat and fifteen barley accessions used in this study were obtained from the National Centre of Agriculture Research and Extension/Jordan (Table 1). The accessions were separately sown in three replicates distributed randomly in three blocks in a field in Al-Qasr-Karak (31º11"N and 35º42"E, altitude of 980 m). A randomized complete block design with three replications of each of the 42 wheat and barley accessions were used. Each replicate represented by a row of 2 m in length and was sown with 30 g of seeds at a depth of 5 cm. Spacing between rows was 0.5 m and 2 m between blocks. Normal cultural practices were followed as practiced by farmers usually do except for no pesticide usage. The field site is characterized by semi-arid conditions with warm weather. The temperature increases gradually during the spring months while the pest larvae are active. A rainfall of 300 mm as long-term annual average was recorded from Al-Rabeh Metrological Station (5 km south of the experimental site). At the end of March, three researchers independently estimated the percentage of infestation for all the accessions for the two successive cropping seasons; 2011/12 and 2012/13. At the same time, number of larvae per plant was recorded for each accession. In addition, yield grain and straw dry mass of all accessions were separately recorded at the harvesting time. Three correlations were linearly plotted; the first two were between the infestation level versus both of yield grain and straw dry mass, and the third one was between larval population size and straw dry mass.
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Laboratory experiment. A laboratory experiment was conducted to quantify the fourth relationship between the larval population size and leaf area consumed by such size. Eighty wheat and thirty-eight barley accessions were used in the experiment (Table 2). Seeds of each wheat and barley accession were sown in pots of 50 cm in height and 30 cm in diameter. The pots were kept under a normal field conditions at the Faculty of Agriculture, Mutah' University, Al-Rabeh-Karak. For the experiment, four trays (100 cm in length and 40 cm in width) were used; representing four replicates; where layer of cotton was spread in the tray bottom and wetted as needed. A piece of leaf cut of 10 cm2 area of each of 80 wheat accessions and 38 of barley accessions were made when plants were about 15 cm in length was laid down into each tray. Hereafter, a total of 200 S. temperatella in the third larval instars were introduced per tray. The trays were kept under laboratory conditions of 20 ± 5 °C temperature, 50 ± 10% relative humidity and 12:12 (L: D) h photoperiod. After 3-day-period, the number of larvae attacked each accession leaf-strip and their associated leaf areas consumed were recorded. Data obtained for both variables (number of larvae and leaf area consumed) were plotted for both crops.
Statistical analysis. The statistical analysis was performed using the proc GLM of the statistical package SigmaStat version 16.0 (SPSS 1997). Regressions' analysis between the infestation level versus both of yield grain and straw dry mass, as well as between larval population size versus both of straw dry mass and leaf area consumed was conducted using Spearman's correlation method (Zar 1999).
]]> Results
The results showed that none of the cultivars and/or accessions tested of wheat and barley was immune to S. temperatella attack. The linear regression between percentage of infestation and grain yield was a negative function; this means that at high percentages of infestation, the grain yield is low (Fig. 1A and B). This correlation was significant for both of wheat (r = -0.405, P = 0.036) and barley (r = -0.503, P = 0.050) (Table 3). The minimum percentages of infestation for wheat and barley were 10.6 and 13.3%, in which accessions produced 0.21 and 0.15 g of seeds/plant, respectively, while the maximum percentages of infestation were 33.0 and 38.1% producing 0.44 and 0.29 g of seeds/plant for both crops, respectively.
The correlation of infestation percentage versus straw dry mass is also of negative function for both crops (Figs. 2A and B); in which the higher foliage infestation by larvae reduced the straw dry mass much more than the lower infestation. The maximum percentages of infestation for wheat and barley resulted in straw production of 2.03 and 1.10 g/plant as compared to 1.56 and 1.00 g of straw/plant that obtained at the minimum infestation percentages, respectively. Slight negative functions were appeared between the number of larvae that attack plant foliage and the straw dry mass (Figs. 3A and B), in which the larval feeding is slightly reduced plant foliage. This inverse correlation is not significant for wheat (r = -0.212, P = 0.288) and also barley (r = -0.304, P = 0.271) as shown in Table 3.
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A positive function was found, that is between the number of larvae and leaf area consumed by the pest larvae (Figs. 4 A and B); in which the increasing number of larvae the increased leaf area consumed. This function was highly significant for both of wheat (r = 0.965, P = 0.000) and barley (r = 0.974, P = 0.000) (Table 3). By using the models obtained, one 3rd instar larva consumed during 3 days about 0.30 cm2 leaf area of wheat and about 0.38 cm2 of barley; representing 3.0-3.8% of the leaf area provided, respectively.
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Discussion
Due to the importance of wheat and barley crops in food security and also due to the increasing demand for their products as food for humans and animals because of the large increase in the world population, it was necessary to investigate and predict the damage caused by any pest threatening such crops. Therefore, this study is found dealing with constructing equations representing damage caused by S. temperatella to hundreds of wheat and barley accessions including the most common cultivars grown locally and regionally; where these cultivars vary in their susceptibility (Al-Zyoud et al. 2009). Larvae of this pest feed strictly on the leaf tissues, which affects plant growth and thereby, the production of grain yield and straw biomass. Therefore, it was a priority to link the pest injury level with yield reduction (Stansly et al. 1996). However, the current study indicated that the average percentage of infestation for the 27 wheat and 15 barley accessions was 22 and 26%, respectively. By applying these infestation percentages on the obtained models that correlated the infestation with the grain yield, the grain yield will be 0.39 and 0.26 g/plant for wheat and barley, respectively. At zero infestation, the yield is estimated at 0.61 and 0.52 g/plant, for wheat and barley, respectively. So that, the overall yield reduction due to the leafminer will be around 36.1% for wheat and 50% for barley, but the individual reduction ranged from 17.4% (in the most resistant wheat accession at the 10.6% infestation) to 73.3% (in the most susceptible barley accession at the 38.1% infestation).
At the average percentage of infestation for wheat (22%) and barley (26%) accessions, and by applying the obtained models that correlated the infestation with straw dry mass, at these averages, the straw yield will be 1.55 and 1.32 g/plant for wheat and barley, respectively. At zero infestation, the straw yield is estimated at 2.21 and 1.58 g/plant, for wheat and barley, respectively. Therefore, the average straw biomass reduction due to the leafminer will be 29.9% for wheat and 16.5% for barley, while the individual reduction was between 8.4% (in the most resistant barley accession at the 13.3% infestation) and 44.8% (in the most susceptible wheat accession at the 33% infestation). The capacity of the cereal leafminer to reduce yield has been documented by many researchers. In Turkey, it was estimated to be 40-60% reduction (Kaya 1976) and later, Duran et al. (1979) estimated the reduction in the field to be around 22%. In Iraq, crop loses caused by the pest on wheat ranged between 10% and 20% (Abu-Yaman 1971). However, several factors may explain the variation of the current study as compared to the previous findings. The most important is that the results of the other researchers representing specific wheat and barley cultivars, while we provide representative numbers for more than 40 accessions and cultivars. Other factors can explain such variation is the differences in the environmental conditions, mainly rainfall which is scarce in our experimental location and maximized the pest infestation. Highly significant leafminer injury to wheat and barley in Jordan allows grouping the pest with the leaf-mass consumer injury guilds as proposed by Peterson (2001).
Models obtained for the number of larvae versus the leaf area consumed showed that one third larval instar of S. temperatella consumed during 3 days an average of 0.3 cm2 (3%) of the leaf area of wheat and 0.38 cm2 (3.8%) of the barley of the offered leaves. Here, leaf area consumed was considered as a criterion to measure the larval damage and not the number of mines, as often number of mines does not give a clear indication of the damage. Peiia and Schaffer (unpublished data) found a poor correlation between the number of mines and the percentage of leaf damage; this was presumably due to the large variability in larval size and instars in a single leaf. Leaf area consumed by other leafminers is found by Knapp et al. (1995), in which one citrus leafminer larva could consume a leaf area between 1-7 cm2 during the period of the entire larval stage (10-19 days). Our finding of the leaf area consumed/larva in 3 days is in agreement with the finding of Knapp et al. (1995), and contrasts that one reported by Sugiura and Yamazaki (2003), in which on average, one larva of the sawfly leafminer Profenusa japonica consumed 71% of the total area of a leaflet. At the average number of larvae/plant of the 27 wheat (3.15) and the 15 barley (2.60) accessions, and also by applying the models that correlated the number of larvae with straw dry mass, the straw biomass is estimated at 1.66 and 1.29 g/plant for wheat and barley, respectively. At the average number of larvae/ plant of the 27 wheat accessions (3.15) and of the 15 barley accessions (2.6), these larvae caused 14.9 and 12.3% reductions in the straw biomass, respectively.
In conclusion, the current study provides equations for predicting the loss due to leaf damage caused by S. temperatella larvae representing 27 wheat and 15 barley accessions. The models quantifying the grain yield loss at (x) percentage of infestation is (y = -0.01x + 0.61) for wheat and is (y = -0.01x + 0.52) for barley. The models quantifying the straw yield loss at (x) percentage of infestation is (y = -0.03x + 2.21) for wheat and is (y = -0.01x + 1.58) for barley. The average percentage of infestation of 22% (wheat) and 26% (barley) reduced the grain yield by 36.1% and 50%, respectively, and reduced the straw dry mass by 29.9% for wheat and 16.5% for barley. The leafminer damage for more than 40 accessions and/or cultivars of wheat and barley is larger for the grain yield than for the straw yield; which clarify the economic importance of the pest.
]]> Acknowledgements
The authors express sincere thanks to the Scientific Research Support Fund at the Ministry of Higher Education and Scientific Research/Jordan for providing financial support for this study.
Literature cited
ABU-YAMAN, I.K. 1971. Control of the wheat leafminer Syringopais temperatella Led. in Iraq. Journal Plant Disease Protection 68: 322-326. [ Links ]
AL-ZYOUD, F. 2012. Effect of field history on the cereal leafminer Syringopais temperatella Led. (Lepidoptera: Scythrididae) and its preference to different wheat and barley cultivars. Pakistan Journal Biological Science 15: 177-185. [ Links ]
AL-ZYOUD, F.; AL-EMIRI, N.; AL-OMARI, A. 2011. Efficacy of the insecticidal bacterium Bacillus thuringiensis (Berliner) (Bt) against the cereal leafminer Syringopais temperatella Led. (Lep., Scythrididae) under laboratory conditions. Jordan Journal Agricultural Sciences 7: 83-92. [ Links ]
]]>AL-ZYOUD, F.; SALAMEH, N.; GHABEISH, I.; SALEH, A. 2009. Susceptibility of different cultivars of wheat and barley to cereal leafminer Syringopais temperatella Led. (Lep., Scythrididae) under laboratory conditions. Journal Food, Agriculture Environment 6: 235-238. [ Links ]
AL-ZYOUD, F. 2008. Effects of direct spray and residual exposure of different insecticides on the cereal leafminer Syringopais temperatella Led. (Lep., Scythrididae) under laboratory conditions. Jordan Journal Agricultural Sciences 4: 1-11. [ Links ]
DURAN, M.; ALTINAYAR, G.; KOYUNCU, N.; DORTBUDAK, Y. 1979. Investigations on survival in the soil, crop loss and control measures relating to the cereal leafminer Syringopais temperatella Led. in a cereal field in Isparta. Bitki Koruma Bulteni 19: 1-15. [ Links ]
GOZUUACIK, C.; ERDOGAN, O.; BEYARSLAN, A. 2008.Syringopais temperatella Led., 1855 and its parasitoids in wheat and barley fields in the southeast Anatolian region of Turkey. Phytoparasitica 36: 489-490. [ Links ]
JEMSI, G.; RADJABI, G. 2003. Study on harvesting agronomic measures and effect of chemical application in controlling the cereal leafminer Syringopais temperatella Led. (Lep., Elachistidae) in Khuzestan province. Applied Entomology Phytopathology 70: 45-61. [ Links ]
]]>JEMSI, G.; SHOJAI, M.; RADJABI, G.; OSTOVAN, H. 2002. Study on economic population dynamic, biology, host range and economic threshold of cereal leafminer in Khuzestan. Journal Agricultural Science 8: 1-21. [ Links ]
JORDAN STATISTICAL YEARBOOK. 2011. Department of Statistics/Annual Agriculture Surveys. Pp 73. Available in: http://www.dos.gov.jo/dos_home_a/main/cd_yb2011/pdf/agri.pdf. [Review date: September 2013] [ Links ]
KAYA, O. 1976. The cereal leafminer Syringopais temperatella. Report, CENTO Scientific Program pp 49-50. [ Links ]
KLAPPERICH, J. 1968. Entomologisch und wirtschaftlich bedeutsame Schadinsekten in der jordanischen Landwirtschaft. Journal Pest Science 41: 164-168. [ Links ]
KNAPP, J .L.; ALBRIGO, L. G.; BROWNING, H. W.; BULLOCK, R. C.; HEPPNER, J. B.; HALL, D. G.; HOY, M. A.;NGUYEN, R.; PENA, J. E.; STANSLY, P. A. 1995. Citrus leafminer Phyllocnistis citrella Stainton: current status in Florida. Gainesville, Florida Cooperative Extension Service, IFAS, University of Florida, pp26. [ Links ]
]]>PETERSON, R. K. D. 2001. Photosynthesis, yield loss, and injury guilds. pp. 83-98. In: Peterson, R.K.D.; Higley, L. G. (Eds.). Biotic stress and yield loss. Boca Raton: CRC, 2001. Chapter 6. [ Links ]
SPSS, Statistical Product and Service Solutions Inc. 1997.SIGMASTAT 2.03: SigmaStat Statistical software user's manual. Chicago, United States. [ Links ]
STANSLY, P. A.; ALBRIGO, L. C.; ROUSE, R. E. 1996. Impact of citrus leafminer on growth and yield of orange and grapefruit. Pp. 47-48. In: Hoy, M. (Ed.). Managing the citrus leafminer. Proceedings of the International conference, 23-25 April 1996, Orlando, FL. Univ. of Florida, Gainesville. [ Links ]
SUGIURA, S.; YAMAZAKI, K. 2003. Host plant, oviposition behavior and larval ecology of a sawfly leafminer, Profenusa japonica (Hymenoptera: Tenthredinidae). Entomology Science 6: 247-251. [ Links ]
ZAR, J. 1999. Bio-statistical analysis. Prentice Hall, Upper Saddle River, NJ 663 p. [ Links ]
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Received: 23-Oct-2013
Accepted: 3-Sep-2014
