<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0120-0488</journal-id>
<journal-title><![CDATA[Revista Colombiana de Entomología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Colomb. Entomol.]]></abbrev-journal-title>
<issn>0120-0488</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Colombiana de Entomología]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0120-04882020000100014</article-id>
<article-id pub-id-type="doi">10.25100/socolen.v46i1.10164</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Macromorfología y crecimiento radial de cepas de hongos entomopatógenos suplementado con polvo de lepidópteros]]></article-title>
<article-title xml:lang="en"><![CDATA[Macromorphology and radial growth of entomopathogenic fungi strains suplement with lepidoteran powder]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Gutiérrez]]></surname>
<given-names><![CDATA[Cipriano]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Guajardo]]></surname>
<given-names><![CDATA[Marina Isabel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vejar-Cota]]></surname>
<given-names><![CDATA[Guadalupe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Meza-García]]></surname>
<given-names><![CDATA[Lorenzo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chávez-Medina]]></surname>
<given-names><![CDATA[Jesús Alicia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Politécnico Nacional  ]]></institution>
<addr-line><![CDATA[Guasave Sinaloa]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma de Occidente  ]]></institution>
<addr-line><![CDATA[Guasave Sinaloa]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Politécnico Nacional  ]]></institution>
<addr-line><![CDATA[Guasave Sinaloa]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Autónoma de Occidente  ]]></institution>
<addr-line><![CDATA[Guasave Sinaloa]]></addr-line>
<country>México</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Instituto Politécnico Nacional  ]]></institution>
<addr-line><![CDATA[Guasave Sinaloa]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2020</year>
</pub-date>
<volume>46</volume>
<numero>1</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-04882020000100014&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0120-04882020000100014&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0120-04882020000100014&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Se estudió la macromorfología de cepas de Beauveria bassiana (B1), Metarhizium anisopliae (M1) e Isaria javanica (HPI-210) y se determinó su crecimiento radial a temperaturas de 25, 28, 30, 35 y 40 °C en medio PDA suplementado con polvos de Diatraea considerata, Spodoptera frugiperda y Galleria mellonella. Los hongos se inocularon adicionando 2 µl de una suspensión con 1 x 107 esporas/ml. Después de 15 días se midió el crecimiento radial de las cepas en mm/d. Para determinar la interacción entre las tres cepas con el polvo de los tres insectos más el control PDA y las cinco temperaturas, se usó un análisis factorial 3 x 4 x 5; los datos del crecimiento de los hongos fueron analizados mediante un ANOVA y una prueba de Tukey. Se encontraron diferencias estadísticas significativas en el crecimiento a diferente temperatura, respecto al control; el crecimiento óptimo en los tres hongos fue a 28 °C, la cepa B1 suplementada con polvo de D. considerata creció 2,76 ± 0,06 mm/d, M1 con G. mellonella 2,77 ± 0,02 mm/d, y HPI-210 con D. considerata 3,27 ± 0,06 mm/d. La cepa B1 creció 0,45 ± 0,03 mm/d, M1 1,22 ± 0,0 mm/d a 30 °C, mientras que HPI-210 no creció después de 28 °C. Estos resultados sugieren que las tres cepas fueron inducidas a crecer mejor por efecto de los medios suplementados con los insectos; a 28 °C, HPI-210 presentó el mayor crecimiento/d (F = 25,24, E8, P = 0,0001) seguido de M1 y B1, mientras que M1 con suplemento de S. frugiperda creció mejor a 30 °C (F = 38,33, E8, P = 0,0001). Estos resultados de crecimiento radial, desarrollo macromorfológico y tolerancia a la temperatura permiten seleccionar a las mejores cepas para evaluar su virulencia en insectos plaga.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The macromorphology of Beauveria bassiana (B1), Metarhizium anisopliae (M1) and Isaria javanica (HPI-210) strains was studied, as well as its radial growth at temperatures of 25, 28, 30, 35 and 40 °C in PDA medium supplemented with powder of Diatraea considerata, Spodoptera frugiperda and Galleria mellonella. The fungi were inoculated by adding 2 µl of a suspension with 1 x 107 spores/ml. Afterwards the radial strains growth in mm/d was measured during 15 d. To determine the interaction between the three strains, the three lepidopteran powders, control PDA and the five temperatures, a factorial analysis 3 x 4 x 5 was used; fungi growth data was analyzed by ANOVA and Tukey test. The macromorphological characteristics of each species were studied, finding significant statistical differences in the growth at different temperatures, with respect to the control. The optimal growth for the three fungi was at 28 °C. Strain B1 supplemented with D. considerata powder grew 2.76 ± 0.06 mm/d, M1, G. mellonella 2.77 ± 0.02 mm/d and M1 HPI-210 D. considerata 3.27 ± 0.06 mm/d. Strains B1 grew 0.45 ± 0.03 mm/d and M1 1.22 ± 0.03 at 30 °C, while HPI-210 did not grow after 28 °C. These results suggested that the strains were induced by medium contained insect supplementation; at 28 °C, HPI-210 obtained the best growth/d (F = 25.24, E8, P = 0.0001), followed by M1 and B1, while M1 supplemented with S. frugiperda grew best at 30 °C (F = 38.33, E8, P = 0.0001). These results of radial growth, macromorphology and tolerance of strains at temperature, allow the selection of the best strains to assess its virulence in insect pests.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Crecimiento radial]]></kwd>
<kwd lng="es"><![CDATA[entomopatógenos]]></kwd>
<kwd lng="es"><![CDATA[hongos]]></kwd>
<kwd lng="es"><![CDATA[Lepidoptera]]></kwd>
<kwd lng="es"><![CDATA[macromorfología]]></kwd>
<kwd lng="es"><![CDATA[medio suplementado]]></kwd>
<kwd lng="en"><![CDATA[Growth fungi]]></kwd>
<kwd lng="en"><![CDATA[entomopathogens]]></kwd>
<kwd lng="en"><![CDATA[fungi]]></kwd>
<kwd lng="en"><![CDATA[Lepidoptera]]></kwd>
<kwd lng="en"><![CDATA[macromorphology]]></kwd>
<kwd lng="en"><![CDATA[medium supplement]]></kwd>
</kwd-group>
</article-meta>
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