<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0120-100X</journal-id>
<journal-title><![CDATA[Revista ION]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. ion]]></abbrev-journal-title>
<issn>0120-100X</issn>
<publisher>
<publisher-name><![CDATA[Universidad Industrial de Santander]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0120-100X2017000100091</article-id>
<article-id pub-id-type="doi">10.18273/revion.v30n1-2017007</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Producción de Ácidos Grasos Poliinsaturados a partir de Biomasa Microalgal en un Cultivo Heterotrófico]]></article-title>
<article-title xml:lang="en"><![CDATA[Production of Polyunsaturated Fatty Acids from Microalgal Biomass in Heterotrophic Culture]]></article-title>
<article-title xml:lang="pt"><![CDATA[Produção de Ácidos Gordos Poli-Insaturados a partir de Biomassa de Microalgas na Cultura Heterotróficos]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Leal Medina]]></surname>
<given-names><![CDATA[Gloria Inés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Abril Bonett]]></surname>
<given-names><![CDATA[José Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez Gélvez]]></surname>
<given-names><![CDATA[Silvia Juliana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Muñoz Peñaloza]]></surname>
<given-names><![CDATA[Yaneth Amparo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Peñaranda Lizarazo]]></surname>
<given-names><![CDATA[Elena María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Urbina Suárez]]></surname>
<given-names><![CDATA[Néstor Andrés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Francisco de Paula Santander (UFPS) Ingeniería Biotecnológica ]]></institution>
<addr-line><![CDATA[Cúcuta ]]></addr-line>
<country>Colombia</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Francisco de Paula Santander (UFPS) Departamento de Medio Ambiente ]]></institution>
<addr-line><![CDATA[Cúcuta ]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2017</year>
</pub-date>
<volume>30</volume>
<numero>1</numero>
<fpage>91</fpage>
<lpage>103</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-100X2017000100091&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0120-100X2017000100091&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0120-100X2017000100091&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El trabajo aquí presentado se enfocó en la producción de ácidos grasos poliinsaturados o PUFA&#8217;s (por sus siglas del inglés PolyUnsaturated Fatty Acids) a partir de biomasa microalgal en un cultivo heterotrófico. Para esto, se utilizaron las algas Chlorella sp. y Scenedesmus sp., en condiciones heterotróficas, posteriormente se seleccionó la cepa con mayor productividad, se realizaron las cinéticas con ambas algas para cuantificar la concentración de biomasa, glucosa, nitrógeno y fósforo; se extrajeron los lípidos y se analizaron por cromatografía de gases. El cultivo heterotrófico se estableció en un reactor de tanque agitado de flujo continuo o CSTR (por sus siglas del inglés Continuous Stirred Tank Reactor) de 1L, con las siguientes condiciones; 28°C, 1vvm, pH 6,8 y relación C/N 12:1. Luego, se realizó el cultivo en un &#8220;Biorreactor BioFlo 115&#8221; con volumen de 10L y se determinó la productividad de los lípidos obtenidos. El perfil lipídico permitió establecer que el ácido graso obtenido en mayor cantidad en CHL2 es el ácido oleico (C 18:1) con un porcentaje igual al 28,75 del total de ácidos grasos, también se destacan la acumulación de los ácidos grasos palmitoléico (C 16:1) con 19,75%, ácido araquídico (C 20:0) con 19,37%, ácido linoleico (C 18:2) con 11,86%, ácido palmítico (C 16:0) con 7,24%, ácido linolénico (&#612;-C 18:3) con 2,61%, ácido erúcico (C 22:1) con 4,61% y ácido esteárico (C 18:0) 2,4%.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract In this research, we obtained Polyunsaturated Fatty Acids (PUFAs) from Microalgal Biomass in heterotrophic culture. In order to obtain these PUFA&#8217;s we used Chlorella sp. and Scenedesmus sp. strains with heterotrophic conditions. Subsequently, we selected the strain with higher productivity and determined the growth kinetics and biomass, glucose, phosphorus and nitrogen yields and the lipids extracted analyzed by gas chromatography. The heterotrophic culture was established in 1L Continuos Stirred-Tank Reactor (CSTR) under specific conditions; 28°C, 1vvm, pH 6,8 and a relation C/N 12:1. Then, we performed sub-cultivation in a &#8220;Bioreactor BioFlo115&#8221; with 10L volume and made productivity analysis of lipids. Lipids profile allowed us to determine that higher Fatty Acid present in Chlorella sp. is oleic acid (C 18:1) and represented a percentage of 28,75 of total fatty acids. Also we noted the accumulation the other fatty acids like; palmitoleic acid (C 16:1) 19.75%, arachidonic acid (C 20:0) 19.37%, linoleic acid (C 18:2) 11.86%, palmitic acid (C 16:0) 7.24%, linoleic acid (&#612;-C 18:3), 2.61%, erucic acid (C 22:1) 4.61%, stearic acid (C 18:0) 2.4%.]]></p></abstract>
<abstract abstract-type="short" xml:lang="pt"><p><![CDATA[Resumo O trabalho aqui apresentado voltada para a produção de ácidos graxos poliinsaturados ou PUFAs (por sua sigla em ácidos graxos poliinsaturados Inglês) a partir da biomassa de microalgas em uma cultura heterotróficos. Para isso, foram utilizados algas Chlorella sp. e Scenedesmus sp., em condições heterotróficas, em seguida, a tensão com o aumento da produtividade foi seleccionado, as cinéticas foram realizadas tanto com as algas para quantificar a concentração de biomassa, da glicose, de azoto e de fósforo; lípidos foram extraídos e analisados por cromatografia em fase gasosa. A cultura foi criada heterotrófica em um reactor de tanque agitado de fluxo contínuo (CSTR) de 1L, com as seguintes condições; 28°C, 1vvm, pH 6,8 e C/N rácio de 12:1. Em seguida, a cultura foi realizada num &#8220;Biorreactor BioFlo115&#8221; com um volume de 10L e produtividade de lípidos obtidos foi determinada. O perfil lipídico estabelecido que o ácido graxo obtido em maiores quantidades em CHL2 é ácido oleico (C 18:1), com uma percentagem igual a 28,75 do total de ácidos graxos, também destaca a acumulação de ácidos graxos palmitoléico (C 16:1), com 19,75%, ácido araquídico (C 20:0), com 19,37%, ácido linoleico (C 18:2) 11,86%, ácido palmítico (C 16:0) 7, 24%, ácido linolénico (&#612;-C 18:3) com 2,61% de ácido erúcico ( C 22:1) com 4,61% de ácido esteárico e (C 18:0) 2,4%.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[ácidos grasos poliinsaturados (PUFA&#8217;s)]]></kwd>
<kwd lng="es"><![CDATA[bomasa microalgal]]></kwd>
<kwd lng="es"><![CDATA[Chlorella sp.]]></kwd>
<kwd lng="es"><![CDATA[Scenedesmus sp.]]></kwd>
<kwd lng="en"><![CDATA[PUFA&#8217;s]]></kwd>
<kwd lng="en"><![CDATA[microalgal biomass]]></kwd>
<kwd lng="en"><![CDATA[Chlorella sp.]]></kwd>
<kwd lng="en"><![CDATA[Scenedesmus sp.]]></kwd>
<kwd lng="pt"><![CDATA[ácidos graxos poliinsaturados (PUFAs)]]></kwd>
<kwd lng="pt"><![CDATA[biomassa de microalgas]]></kwd>
<kwd lng="pt"><![CDATA[Chlorella sp]]></kwd>
<kwd lng="pt"><![CDATA[Scenedesmus sp]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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