<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0120-2812</journal-id>
<journal-title><![CDATA[Acta Agronómica]]></journal-title>
<abbrev-journal-title><![CDATA[Acta Agron.]]></abbrev-journal-title>
<issn>0120-2812</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional de Colombia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0120-28122017000400598</article-id>
<article-id pub-id-type="doi">10.15446/acag.v66n4.60080</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Ex situ potential conservation of ipecacuanha ( Psychotria ipecacuanha (Brot.) Stokes . ) , a critically endangered medicinal plant species]]></article-title>
<article-title xml:lang="es"><![CDATA[Potencial de conservación ex situ de ipecacuana ( Psychotria ipecacuanha (Brot.) Stokes .), una planta medicinal en peligro crítico de extinción]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Urrea Trujillo]]></surname>
<given-names><![CDATA[Aura Inés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Botero Giraldo]]></surname>
<given-names><![CDATA[Catalina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Naranjo Gómez]]></surname>
<given-names><![CDATA[Esther Julia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,aff1  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<volume>66</volume>
<numero>4</numero>
<fpage>598</fpage>
<lpage>605</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-28122017000400598&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0120-28122017000400598&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0120-28122017000400598&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour of developed buds and was initially performed at 3 months and then every two months until 12 months. The explants conserved for 9 months in culture medium without mannitol osmotic regulator and temperature of 18°C, showed response after transferring and reculturing in recovery culture medium for 6-8 weeks, this process had not achieved any effect on plant growth and vigour in the recovery phase. In addition, the critical parameters in the acclimatization process were determined.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Psychotria ipecacuanha es una especie medicinal amenazada en Colombia, debido a la explotación indiscriminada que varias décadas atrás sufrió por su importancia terapéutica. Su gran valor medicinal se debe a la producción de emetina y sus derivados, principalmente en las raíces. Ante este panorama, el presente estudio tuvo como objetivo evaluar diferentes parámetros para la conservación in vitro de P. ipecacuanha por crecimiento limitado, usando como explantes nudos de plantas in vitro. Se evaluó la respuesta de crecimiento a 18, 23 y 25°C sin y con la adición al medio de cultivo basal MS del regulador osmótico manitol a 15 y 30 gL-1. El registro de los datos de supervivencia (%), altura (cm), desarrollo de brotes axilares, número de hojas, formación de callo y vigor de los brotes desarrollados, se realizó inicialmente a los 3 meses y luego cada dos meses hasta completar 12 meses. Una temperatura de 18°C, sin la adición del regulador osmótico manitol al medio cultivo basal MS, permitió mantener vitroplantas de esta especie hasta por nueve meses, sin verse afectado el crecimiento y vigor en la etapa de recuperación. Además se logró determinar los parámetros críticos en el proceso de aclimatización.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[In vitro conservation]]></kwd>
<kwd lng="en"><![CDATA[plant limited growth]]></kwd>
<kwd lng="en"><![CDATA[osmotic regulator]]></kwd>
<kwd lng="en"><![CDATA[recovery phase]]></kwd>
<kwd lng="en"><![CDATA[vigour]]></kwd>
<kwd lng="es"><![CDATA[Conservación in vitro]]></kwd>
<kwd lng="es"><![CDATA[crecimiento limitado]]></kwd>
<kwd lng="es"><![CDATA[etapa de recuperación]]></kwd>
<kwd lng="es"><![CDATA[regulador osmótico]]></kwd>
<kwd lng="es"><![CDATA[vigorosidad]]></kwd>
</kwd-group>
</article-meta>
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