<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0123-3475</journal-id>
<journal-title><![CDATA[Revista Colombiana de Biotecnología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. colomb. biotecnol]]></abbrev-journal-title>
<issn>0123-3475</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Biotecnología, Universidad Nacional de Colombia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0123-34752015000100032</article-id>
<article-id pub-id-type="doi">10.15446/rev.colomb.biote.v17n1.</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Optimization of in vitro conservation of Dioscorea spp germplasm by minimal growth]]></article-title>
<article-title xml:lang="es"><![CDATA[Optimización de la conservación in vitro de germoplasma de Dioscorea spp por crecimiento mínimo]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz Narváez]]></surname>
<given-names><![CDATA[Lucía Candelaria]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Carmona Wilches]]></surname>
<given-names><![CDATA[Oscar Elías]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Beltrán Herrera]]></surname>
<given-names><![CDATA[Javier Darío]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Sucre  ]]></institution>
<addr-line><![CDATA[Sincelejo Sucre]]></addr-line>
<country>Colombia</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Sucre  ]]></institution>
<addr-line><![CDATA[Sincelejo Sucre]]></addr-line>
<country>Colombia</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de Sucre  ]]></institution>
<addr-line><![CDATA[Sincelejo Sucre]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>01</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>01</month>
<year>2015</year>
</pub-date>
<volume>17</volume>
<numero>1</numero>
<fpage>32</fpage>
<lpage>39</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0123-34752015000100032&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0123-34752015000100032&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0123-34752015000100032&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Dioscorea alata cv. "Criollo" and Dioscorea rotundata cv. "Espino" are constituted as the two cultivars mostly cultivated in the department of Sucre, Colombia. For this reason the University of Sucre has been implementing tissue culture techniques for their multiplication throughout in vitro propagation. However, this methodology support accessions for a period about 4 months, causing continuous subcultures, increased costs and labor. Therefore the objective of the present investigation was to establish an optimal culture media for in vitro minimal growth conservation of different accessions (D. alata and D. rotundata) from the University of Sucre yam's genebank , for a period of 8 months by modifying the basic MS culture medium. The following osmolytes were used: sucrose, mannitol and sorbitol in different percentages (S:M:S). Eight treatments (T) in the following percentages were evaluated: T1 (control) (3:0:0), T2 (0:1,5:0), T3(0:0:2), T4 (0:1,5:2), T5 (0:0:1) y T6 (0:0:3), T7 (0:1,5:1) y T8 (0:1,5:3). Every 30 days data was recorded for: survival (%), expanded leaves (%), length of stem and roots, number of leaves and roots, phenolization (%), leaf senescence (%) and callus presence (%). The best results for D. alata and D. rotundata, were observed in treatment T4 (0:1,5:2), where a high percentage of survival evidence, a minimum percentage of leaf senescence and a limited response for the other variables. Therefore, these results indicate the potential of this media, to increase 100% in vitro growth conservation over the conventional media, and ensuring the viability and normal development of the accessions for more than four months period.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El ñame Criollo (Dioscorea alata) y el ñame Espino (Dioscorea rotundata) se constituyen como las dos especies mayormente cultivadas en el departamento de Sucre, Colombia. Por esta razón en la Universidad de Sucre se han implementado técnicas para lograr su conservación mediante la propagación in vitro, sin embargo esta metodología conserva las accesiones por un periodo no mayor a los 4 meses, provocando continuos subcultivos, aumento de costos y mano de obra. Por ello la presente investigación tuvo como objetivo establecer medios de cultivo óptimos para la conservación in vitro por crecimiento mínimo de diferentes accesiones (D. alata y D. rotundata) pertenecientes al banco de germoplasma de la Universidad de Sucre, durante un periodo de 8 meses. Esto mediante la modificación del medio de cultivo base MS; con los siguientes osmolitos: sacarosa, manitol y sorbitol. Se avaluaron 8 tratamientos (T) en los siguientes porcentajes T1 (control) (3:0:0), T2 (0:1,5:0), T3 (0:0:2), T4 (0:1,5:2), T5 (0:0:1) y T6 (0:0:3), T7 (0:1,5:1) y T8 (0:1,5:3). Cada 30 días se evaluó: supervivencia (%), hojas expandidas (%), longitud del tallo y raíz, número de nudos y raíces, oxidación (%), senescencia foliar (%) y callo (%). Los resultados mostraron que las especies D. alata y D. rotundata, se conservan de forma óptima, en la combinación T4 (0:1,5:2), donde se evidencia un alto porcentaje de supervivencia, un mínimo porcentaje de senescencia foliar y un desarrollo restringido en el resto de variables. Garantizando así la disponibilidad y el desarrollo normal de las accesiones en un periodo superior a 4 meses.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[yam]]></kwd>
<kwd lng="en"><![CDATA[mannitol]]></kwd>
<kwd lng="en"><![CDATA[sorbitol]]></kwd>
<kwd lng="en"><![CDATA[sucrose]]></kwd>
<kwd lng="es"><![CDATA[ñame]]></kwd>
<kwd lng="es"><![CDATA[manitol]]></kwd>
<kwd lng="es"><![CDATA[sorbitol]]></kwd>
<kwd lng="es"><![CDATA[sacarosa]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Acedo]]></surname>
<given-names><![CDATA[V.Z.]]></given-names>
</name>
<name>
<surname><![CDATA[Arradoza]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development of in vitro slow growth culture for yam (Dioscorea alata L.)]]></article-title>
<source><![CDATA[PCARRD]]></source>
<year>2006</year>
<volume>30</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Borges]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Alarcón]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Malaurie]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Conservación in vitro de Dioscorea alata L. clon caraqueño (Dioscoreaceae)]]></article-title>
<source><![CDATA[Revista Peruana de Biología]]></source>
<year>2009</year>
<volume>16</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>203-8</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cárdenas]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Villegas]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Potencial osmótico del medio de cultivo con diferentes componentes para la propagación in vitro]]></article-title>
<source><![CDATA[Revista Fitotecnia Mexicana]]></source>
<year>2002</year>
<volume>25</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>213-7</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Carmona]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Díaz]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Beltrán]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Efecto de los osmolitos sacarosa, manitol y sorbitol en la conservación in vitro de Di-oscorea alata, D. bulbifera, D. rotundata y D. trifida por el método de crecimiento mínimo]]></article-title>
<source><![CDATA[Revista Asociación Colombiana de Ciencias Biológicas]]></source>
<year>2013</year>
<numero>25</numero>
<issue>25</issue>
<page-range>1-519</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[De Mendiburu]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
</person-group>
<source><![CDATA[Manual rápido de uso de la librería agricolae]]></source>
<year>2012</year>
<page-range>60</page-range><publisher-name><![CDATA[Universidad Nacional Agraria La Molina]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Estrada]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Borges]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Aplicación de algunas técnicas de estadística multivariada al estudio de la conservación in vitro de germo-plasma de Dioscorea alata L]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Hernández]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Kosky]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Malaurie]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Biotecnología Vegetal]]></source>
<year>2009</year>
<volume>9</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>153-9</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Murashige]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A revised médium for rapid growth and bioassays with tobacco tissue cultures]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Skoog]]></surname>
<given-names><![CDATA[F]]></given-names>
</name>
<name>
<surname><![CDATA[Borges García]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Physiologia plantarum]]></source>
<year>1962</year>
<page-range>203-8</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Neiva]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Jiménez]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Técnicas de conservación in vitro para el establecimiento de bancos de germoplasma en cultivos tropicales]]></article-title>
<source><![CDATA[Agronomía Mesoamericana]]></source>
<year>2010</year>
<volume>21</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>193-205</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Reina]]></surname>
<given-names><![CDATA[Y. C]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[El cultivo de ñame en el Caribe colombiano. Documentos de trabajo sobre economía regional]]></article-title>
<source><![CDATA[Banco de la Republica sucursal Cartagena]]></source>
<year>2012</year>
<volume>1</volume>
<numero>168</numero>
<issue>168</issue>
<page-range>31</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Identificación del agente causal de una bacteriosis en ñame (Dioscorea alata L.)]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Matehus]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Gerstl]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Santana]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Interciencia]]></source>
<year>2008</year>
<volume>33</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>1-11</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ortega]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Beltrán]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Marrugo]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro]]></article-title>
<source><![CDATA[Revista Colombiana De Biotecnología]]></source>
<year>2011</year>
<volume>13</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>33-41</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Salazar]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Beltrán]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Microtuberización en Ñame (Dioscorea alata L.) var. "Pico de Botella"]]></article-title>
<source><![CDATA[Revista Colombiana de Biotecnología]]></source>
<year>2002</year>
<volume>4</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>27-32</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Thurston]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
</person-group>
<source><![CDATA[Tropical plant diseases]]></source>
<year>1998</year>
<edition>Segunda edición</edition>
<page-range>79-82</page-range><publisher-name><![CDATA[APS-Press]]></publisher-name>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
