<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0123-3475</journal-id>
<journal-title><![CDATA[Revista Colombiana de Biotecnología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. colomb. biotecnol]]></abbrev-journal-title>
<issn>0123-3475</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Biotecnología, Universidad Nacional de Colombia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0123-34752019000100101</article-id>
<article-id pub-id-type="doi">10.15446/rev.colomb.biote.v21n1.80248</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Microalgae protoplasts isolation and fusion for biotechnology research]]></article-title>
<article-title xml:lang="es"><![CDATA[Aislamiento y fusión de protoplastos de microalgas para aplicaciones biotecnológicas]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Echeverri]]></surname>
<given-names><![CDATA[Danilo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Romo]]></surname>
<given-names><![CDATA[Juliana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Giraldo]]></surname>
<given-names><![CDATA[Néstor]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Atehortúa]]></surname>
<given-names><![CDATA[Lucía]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Antioquia  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<volume>21</volume>
<numero>1</numero>
<fpage>101</fpage>
<lpage>112</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0123-34752019000100101&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0123-34752019000100101&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0123-34752019000100101&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Protoplasts are microbial or vegetable cells lacking a cell wall. These can be obtained from microalgae by an enzymatic hydrolysis process in the presence of an osmotic stabilizer. In general, protoplasts are experimentally useful in physiological, genetic and biochemical studies, so their acquisition and fusion will continue to be an active research area in modern biotechnology. The fusion of protoplasts in microalgae constitutes a tool for strain improvement because it allows both intra and interspecific genetic recombination, resulting in organisms with new or improved characteristics of industrial interest. In this review we briefly describe the methodology for obtaining protoplasts, as well as fusion methods and the main applications of microalgal platforms.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Los protoplastos son células microbianas o vegetales que carecen de pared celular. Estos pueden obtenerse a partir de microalgas por un proceso de hidrólisis enzimática en presencia de un estabilizador osmótico. En general, los protoplastos son experimentalmente útiles en estudios fisiológicos, genéticos y bioquímicos, por lo que su obtención y fusión continuarán siendo un área de investigación activa en la biotecnología moderna. La fusión de protoplastos en microalgas constituye una herramienta para el mejoramiento de cepas pues permite la recombinación genética intra e interespecífica, logrando así organismos con nuevas características de interés industrial. En esta revisión, describimos brevemente la metodología para obtener protoplastos, métodos de fusión y las principales aplicaciones de las plataformas basadas en microalgas.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Enzymatic hydrolysis]]></kwd>
<kwd lng="en"><![CDATA[microalgae]]></kwd>
<kwd lng="en"><![CDATA[cell wall]]></kwd>
<kwd lng="en"><![CDATA[protoplast fusion]]></kwd>
<kwd lng="en"><![CDATA[strain improvement]]></kwd>
<kwd lng="es"><![CDATA[Hidrolisis enzimática]]></kwd>
<kwd lng="es"><![CDATA[microalga]]></kwd>
<kwd lng="es"><![CDATA[pared celular]]></kwd>
<kwd lng="es"><![CDATA[fusión de protoplastos]]></kwd>
<kwd lng="es"><![CDATA[mejora de la tensión]]></kwd>
</kwd-group>
</article-meta>
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