<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0123-3475</journal-id>
<journal-title><![CDATA[Revista Colombiana de Biotecnología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. colomb. biotecnol]]></abbrev-journal-title>
<issn>0123-3475</issn>
<publisher>
<publisher-name><![CDATA[Instituto de Biotecnología, Universidad Nacional de Colombia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0123-34752019000200022</article-id>
<article-id pub-id-type="doi">10.15446/rev.colomb.biote.v21n2.83410</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[In vitro tissue culture, preliminar phytochemical analysis, and antibacterial activity of Psittacanthus linearis (Killip) J.K. Macbride (Loranthaceae)]]></article-title>
<article-title xml:lang="es"><![CDATA[Cultivo de tejidos in vitro, análisis fitoquímico preliminar y actividad antibacteriana de Psittacanthus linearis (Killip) J.K. Macbride (Loranthaceae)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bailadores Bollona]]></surname>
<given-names><![CDATA[Juan Pablo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Delgado Paredes]]></surname>
<given-names><![CDATA[Guillermo Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Wagner]]></surname>
<given-names><![CDATA[Marcelo Luis]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas Idrogo]]></surname>
<given-names><![CDATA[Consuelo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional Pedro Ruiz Gallo Facultad de Ciencias Biológicas ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Peru</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Nacional Pedro Ruiz Gallo Facultad de Ciencias Biológicas ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Peru</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de Buenos Aires Facultad de Farmacia y Bioquímica ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Argentina</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Nacional Pedro Ruiz Facultad de Ciencias Biológicas ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Perú</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<volume>21</volume>
<numero>2</numero>
<fpage>22</fpage>
<lpage>35</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0123-34752019000200022&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0123-34752019000200022&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0123-34752019000200022&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[ABSTRACT Hemiparasitic plants commonly known as mistletoe (muérdago in Spanish) in the families Santalaceae and Loranthaceae are common in various kinds of plants or trees, and many hemiparasitic plants are used for medicinal purposes in various parts of the world. The objective of the present work, carried out in Psittacanthus linearis (suelda con suelda), a representative species in the seasonally dry forest (SDF) from the north of Perú, was to study aspects of in vitro tissue culture, carry out preliminary phytochemical analysis, and assess antibacterial activity. Seeds of individuals of P. linearis, which used Prosopis pallida (algarrobo) as host plant, were collected and used to induce in vitro seed germination, clonal propagation, callus induction and organogenesis. Stems, leaves and fruits of individuals of P. linearis were dried, powdered, and subjected to ethanol extraction. Posteriorly the extract was first recovered with ethanol and the remnant with chloroform, which formed the ethanolic and chloroformic fraction. A preliminary phytochemical screening was performed and preliminary antibacterial studies with Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were carried out and their results are discussed. This is the first report about in vitro tissue culture, phytochemical analysis and antibacterial activity of P. linearis. The results may have important implications for understanding physiological and biochemical interactions between host and hemiparasitic species as well as P. linearis with P. pallida and other SDF species.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Las plantas hemiparásitas o 'mistletoe' o 'muérdago' son comunes en varios grupos vegetales o árboles, perteneciendo a las familias Santalaceae and Loranthaceae y muchas plantas hemiparásitas son usadas como medicina en varios lugares del mundo. El objetivo del presente trabajo realizado en Psittacanthus linearis or 'suelda con suelda', especie representativa en el bosque estacionalmente seco (BES) del norte del Perú, fue estudiar algunos aspectos en el cultivo de tejidos in vitro, el análisis fitoquímico preliminar y su actividad antibacterial. Semillas de P. linearis teniendo a Prosopis pallida 'algarrobo' como hospedero, fueron colectadas y utilizadas en la germinación in vitro, propagación clonal, inducción de callos y procesos organogénicos. Tallos, hojas y frutos de plantas silvestres fueron secados, pulverizados y sometidos a extracción con etanol y el extracto fue recuperado primero con etanol y el remanente con cloroformo formando las fraciones etanólica y clorofórmica. Se realizó un estudio fitoquímico y antibacteriano preliminar utilizando Staphylococcus aureus, Escherichia coli y Pseudomonas aeruginosa y los resultados son discutidos. Este trabajo es el primer estudio sobre cultivo de tejidos, análisis fitoquímico y actividad antibacteriana de P. linearis. Los resultados obtenidos tienen importantes implicancias para el conocimiento de las interacciones fisiológicas y bioquímicas entre las especies hospederas y las plantas hemiparásitas, como P. linearis con P. pallida y otras especies del BES.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Catechin and cyanidin]]></kwd>
<kwd lng="en"><![CDATA[hemiparasitic plant]]></kwd>
<kwd lng="en"><![CDATA[Prosopis pallida]]></kwd>
<kwd lng="en"><![CDATA['suelda con suelda']]></kwd>
<kwd lng="en"><![CDATA[Staphylococcus aureus]]></kwd>
<kwd lng="es"><![CDATA[Catequina y cianidina]]></kwd>
<kwd lng="es"><![CDATA[planta hemiparásita]]></kwd>
<kwd lng="es"><![CDATA[Prosopis pallida]]></kwd>
<kwd lng="es"><![CDATA['suelda con suelda']]></kwd>
<kwd lng="es"><![CDATA[Staphylococcus aureus]]></kwd>
</kwd-group>
</article-meta>
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