<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0370-3908</journal-id>
<journal-title><![CDATA[Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. acad. colomb. cienc. exact. fis. nat.]]></abbrev-journal-title>
<issn>0370-3908</issn>
<publisher>
<publisher-name><![CDATA[Academia Colombiana de Ciencias Exactas, Físicas y Naturales]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0370-39082020000300828</article-id>
<article-id pub-id-type="doi">10.18257/raccefyn.1021</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Validation of an analytical method by GC-FID for the quantification of styrene and &#945;-methylstyrene]]></article-title>
<article-title xml:lang="es"><![CDATA[Validación de un método analítico por GC-FID para la cuantificación de estireno y &#945;-metilestireno]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Verdugo-Torres]]></surname>
<given-names><![CDATA[Brayan David]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cubillo-Lobo]]></surname>
<given-names><![CDATA[Jairo Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas Sarmiento]]></surname>
<given-names><![CDATA[Hugo Alfonso]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Pedagógica y Tecnológica de Colombia Escuela de Ciencias Químicas ]]></institution>
<addr-line><![CDATA[Tunja ]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2020</year>
</pub-date>
<volume>44</volume>
<numero>172</numero>
<fpage>828</fpage>
<lpage>834</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0370-39082020000300828&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0370-39082020000300828&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0370-39082020000300828&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Styrene and &#945;-methylstyrene are substrates of great interest in asymmetric catalysis. Although they have been widely used, known quantification methodologies are restricted to the use of mass spectrometry detectors and are not validated. In the present work, we developed and validated a reliable method by gas chromatography with a flame ionization detector (GC-FID) for the analysis of non-functionalized olefins (styrene and &#945;-methylstyrene) in a liquid matrix using toluene as the internal standard. We explored validation parameters such as selectivity, linearity, detection limit, quantification limit, precision, and accuracy. The results showed an adequate separation of each olefin under the conditions and range of work implemented (6.83x10-4 mol/L - 4.059x10-3 mol/L). The parameters evaluated are within acceptable values indicating that the validated method is selective, linear, precise, and accurate. This work represents an effort to develop a highly safe, efficient, and validated chromatographic method for the quantification of styrene and &#945;-methylstyrene in liquid matrices for their possible application in the field of resins, plasticizers, and polymers where they are mainly involved.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Estireno y &#945;-metilestireno son sustratos de gran interés en catálisis asimétrica. Aunque han sido ampliamente usados, las metodologías de cuantificación conocidas se restringen al uso de detectores de espectrometría de masas y no se encuentran validadas. En el presente trabajo se desarrolló y validó un método confiable mediante cromatografía de gases con detector de ionización de llama (GC-FID) para el análisis de olefinas no funcionalizadas (estireno y &#945;-metilestireno) en matrices líquidas utilizando el tolueno como patrón interno. Los parámetros de validación evaluados incluyeron selectividad, linealidad, límite de detección, límite de cuantificación, precisión y exactitud. El análisis evidenció una separación adecuada de cada analito de interés bajo las condiciones y el rango de trabajo implementado (6,83x10-4 mol/L - 4,059x10-3 mol/L). Los parámetros evaluados registraron valores dentro de los rangos de aceptación, lo que confirmó que el método validado es selectivo, lineal, preciso y exacto. Este trabajo representa un esfuerzo por desarrollar un método cromatográfico validado seguro y eficiente para la cuantificación de estireno y &#945;-metilestireno en matrices líquidas, y su posible aplicación en el campo de las resinas, plastificantes y polímeros.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Validation]]></kwd>
<kwd lng="en"><![CDATA[Method]]></kwd>
<kwd lng="en"><![CDATA[Styrene]]></kwd>
<kwd lng="en"><![CDATA[&#945;-methylstyrene]]></kwd>
<kwd lng="en"><![CDATA[GC-FID]]></kwd>
<kwd lng="es"><![CDATA[Validación]]></kwd>
<kwd lng="es"><![CDATA[Método]]></kwd>
<kwd lng="es"><![CDATA[Estireno]]></kwd>
<kwd lng="es"><![CDATA[&#945;-metilestireno]]></kwd>
<kwd lng="es"><![CDATA[GC-FID]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aguirre-Ortega]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez-Cuadrado]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Pujol-Forn]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Validación de métodos analíticos]]></source>
<year>2001</year>
<page-range>46</page-range><publisher-loc><![CDATA[Barcelona, España ]]></publisher-loc>
<publisher-name><![CDATA[Asociación Española de Farmacéuticos de la Industria]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="">
<collab>Association of Official Agricultural Chemists - AOAC</collab>
<source><![CDATA[Appendix K: Guidelines for Dietary Supplements and Botanicals]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Berijani]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Morsali]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Hupp]]></surname>
<given-names><![CDATA[J. T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An effective strategy for creating asymmetric MOFs for chirality induction: a chiral Zr-based MOF for enantioselective epoxidation]]></article-title>
<source><![CDATA[Catalysis Science &amp; Technology]]></source>
<year>2019</year>
<volume>9</volume>
<numero>13</numero>
<issue>13</issue>
<page-range>3388-97</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bubeníková]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Bednár]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Gergel&#8217;]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Igaz]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Adsorption Effect of Added Powder Graphite on Reduction of Volatile Organic Compounds Emissions from Expanded Polystyrene]]></article-title>
<source><![CDATA[BioResources]]></source>
<year>2019</year>
<volume>14</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>9729-38</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Coelho]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Ribeiro]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<source><![CDATA[White biotechnology for sustainable chemistry]]></source>
<year>2016</year>
<page-range>249</page-range><publisher-loc><![CDATA[Cambridge ]]></publisher-loc>
<publisher-name><![CDATA[Royal Society of Chemistry]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Even]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Hutzler]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Wilke]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Luch]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Emissions of volatile organic compounds from polymer-based consumer products: comparison of three emission chamber sizes]]></article-title>
<source><![CDATA[Indoor Air]]></source>
<year>2019</year>
<volume>30</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>40-8</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="">
<collab>Food and Drug Administration - FDA</collab>
<source><![CDATA[Bioanalytical Method Validation Guidance for Indus try]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gabhe]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Development And Validation Of Chromatographic Methods For Simultaneous Quantification Of Drugs In Bulk And In Their Formulations: HPLC And HPTLC Techniques]]></source>
<year>2015</year>
<page-range>22-5</page-range><publisher-loc><![CDATA[Hamburg, Germany ]]></publisher-loc>
<publisher-name><![CDATA[Anchor Academic Publishing]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gennari]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Albrizio]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Monteiro]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A GC-FID method to determine styrene in polystyrene glasses]]></article-title>
<source><![CDATA[Food Analytical Methods]]></source>
<year>2012</year>
<volume>5</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>1411-8</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gomes]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[de Pinho]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Pontes]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Ferreira]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Branco]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Remião]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Gas chromatography-ion trap mass spectrometry method for the simultaneous measurement of MDMA (ecstasy) and its metabolites, MDA, HMA, and HMMA in plasma and urine]]></article-title>
<source><![CDATA[Journal Of Chromatography B]]></source>
<year>2010</year>
<volume>878</volume>
<numero>9-10</numero>
<issue>9-10</issue>
<page-range>815-22</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hadian-Dehkordi]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Hosseini-Monfared]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Enantioselective aerobic oxidation of olefins by magnetite nanoparticles at room temperature: a chiral carboxylic acid strategy]]></article-title>
<source><![CDATA[Green Chemistry]]></source>
<year>2016</year>
<volume>18</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>497-507</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Huang]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Ma]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Cai]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Chiral salen Mn (III) immobilized on ZnPS-PVPA through alkoxyl-triazole for superior performance catalyst in asymmetric epoxidation of unfunctionalized olefins]]></article-title>
<source><![CDATA[Journal Of Organometallic Chemistry]]></source>
<year>2019</year>
<volume>886</volume>
<page-range>27-33</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hwang]]></surname>
<given-names><![CDATA[J. B.]]></given-names>
</name>
<name>
<surname><![CDATA[Lee]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Yeum]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Kim]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Choi]]></surname>
<given-names><![CDATA[J. C.]]></given-names>
</name>
<name>
<surname><![CDATA[Park]]></surname>
<given-names><![CDATA[S.-J.]]></given-names>
</name>
<name>
<surname><![CDATA[Kim]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[HS-GC/MS method development and exposure assessment of volatile organic compounds from food packaging into food simulants]]></article-title>
<source><![CDATA[Food Additives &amp; Contaminants: Part A]]></source>
<year>2019</year>
<volume>36</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>1574-83</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="confpro">
<collab>International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use - ICH</collab>
<source><![CDATA[Validation of analytical procedures: text and methodology Q2(R1)]]></source>
<year>2005</year>
<conf-name><![CDATA[ International conference on harmonization]]></conf-name>
<conf-loc>Geneva, Switzerland </conf-loc>
</nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Poole]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Gas chromatography]]></source>
<year>2012</year>
<edition>1st</edition>
<page-range>435-48</page-range><publisher-loc><![CDATA[Amsterdam, Netherland ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nageswara-Rao]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Validation of Analytical Methods]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Stauffer]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Calibration and Validation of Analytical Methods: A Sampling of Current Approaches]]></source>
<year>2018</year>
<page-range>131-41</page-range><publisher-loc><![CDATA[London, United Kingdom ]]></publisher-loc>
<publisher-name><![CDATA[BoD-Books on Demand]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tang]]></surname>
<given-names><![CDATA[X.]]></given-names>
</name>
<name>
<surname><![CDATA[Tang]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Xu]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Wei]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Sun]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Highly enantioselective epoxidation of styrene and &#945;-methylstyrene catalyzed by new doubly-immobilized chiral (salen) Mn(III) catalysts]]></article-title>
<source><![CDATA[Catalysis Communications]]></source>
<year>2008</year>
<volume>10</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>317-20</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="">
<collab>United Nations Office on Drugs and Crime - UNODC</collab>
<source><![CDATA[Guidance for the Validation of Analytical Methodology and Calibration of Equipment used for Testing of Illicit Drugs in Seized Materials and Biological Specimens]]></source>
<year>2009</year>
</nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wong]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Shi]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Organocatalytic Oxidation. Asymmetric Epoxidation of Olefins Catalyzed by Chiral Ketones and Iminium Salts]]></article-title>
<source><![CDATA[Chemical Reviews]]></source>
<year>2008</year>
<volume>108</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>3958-87</page-range></nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Xia]]></surname>
<given-names><![CDATA[Q.]]></given-names>
</name>
<name>
<surname><![CDATA[Ge]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Ye]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Liu]]></surname>
<given-names><![CDATA[Z.]]></given-names>
</name>
<name>
<surname><![CDATA[Su]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Advances in Homogeneous and Heterogeneous Catalytic Asymmetric Epoxidation]]></article-title>
<source><![CDATA[Chemical Reviews]]></source>
<year>2005</year>
<volume>105</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>1603-62</page-range></nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zuas]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[Mulyana]]></surname>
<given-names><![CDATA[M. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Budiman]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Analytical method validation of GC-FID for the simultaneous measurement of hydrocarbons (C2-C4) in their gas mixture]]></article-title>
<source><![CDATA[Revista Colombiana de Química]]></source>
<year>2016</year>
<volume>45</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>22-7</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
