<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1794-2470</journal-id>
<journal-title><![CDATA[Nova]]></journal-title>
<abbrev-journal-title><![CDATA[Nova]]></abbrev-journal-title>
<issn>1794-2470</issn>
<publisher>
<publisher-name><![CDATA[Universidad Colegio Mayor de Cundinamarca]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1794-24702021000200099</article-id>
<article-id pub-id-type="doi">10.22490/24629448.5498</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Producción de astaxantina bajo factores de estrés utilizando un biorreactor a escala de laboratorio de 5 L]]></article-title>
<article-title xml:lang="en"><![CDATA[Astaxanthin production under stress factors using a 5L laboratory scale bioreactor]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Lancheros Díaz]]></surname>
<given-names><![CDATA[Ana Graciela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Díaz Barrera]]></surname>
<given-names><![CDATA[Luis Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Camacho Kurmen]]></surname>
<given-names><![CDATA[Judith Elena]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,CvLAC  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="Af2">
<institution><![CDATA[,CvLAC  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="Af3">
<institution><![CDATA[,CvLAC  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<volume>19</volume>
<numero>37</numero>
<fpage>99</fpage>
<lpage>119</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S1794-24702021000200099&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S1794-24702021000200099&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S1794-24702021000200099&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Introducción. Haematococcus pluvialis es una microalga que produce astaxantina, un beta-caroteno y antioxidante muy usado en la industria. Para obtener una mayor producción de astaxantina se planteó como objetivo utilizar diferentes factores de estrés, en un biorreactor a escala de laboratorio de 5 litros.  Metodología. Se cultivó la microalga en el medio RM, pH 6,8, temperatura 20±2°C, aire filtrado, iluminación con lámparas blancas 20h luz/4h oscuridad, irradianza 70 &#956;E m-2s-1, diferentes concentraciones de acetato de sodio y cloruro de sodio. Se determinó crecimiento celular, cambios morfológicos y cuantificación de astaxantina y clorofila por espectrofotometría. Se realizó un análisis estadístico utilizando ANOVA (95%).  Resultados. Utilizando 0,299 mg/L de acetato de sodio se obtuvo un crecimiento celular de 2,0 x 104 Cel/mL y una concentración de astaxantina de 2,530 &#956;g/mL, mientras que con 1,6 mg/L de acetato de sodio el crecimiento celular fue de 3,5 x 104 Cel/mL y una concentración de astaxantina de 1,9 &#956;g/ml. El tratamiento al cual se le adicionó 1,6 g/L de acetato de sodio y 6,4 g/L de cloruro de sodio presentó la mayor producción astaxantina 7,3 &#956;g/ml. El tratamiento con acetato de sodio 0,320 g/L + cloruro de sodio 1,28 g/L presentó el mayor crecimiento celular con 1,64x105 células/ml.  Conclusión. Esta investigación destaca la importancia de cultivar inicialmente la microalga utilizando el biorreactor Tecferm de 5 litros y después de su fase exponencial someterla a factores de estrés con acetato de sodio y cloruro de sodio lográndose así la mayor producción de astaxantina 7,325 &#956;g/ml.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Introduction. Haematococcuspluvialis is a microalgae that produces astaxanthin, a beta-carotene and antioxidant widely used in industry. In order to obtain a higher production of astaxanthin, the objective was to use different stress factors, in a 5-liter laboratory-scale bioreactor.  Methodology. The microalgae was cultivated in the RM medium, pH 6.8, temperature 20 ± 2°C, filtered air, illumination with white lamps 20h light/4h darkness, irradiance 70 &#956;E m-2s-1, different concentrations of sodium acetate and chloride of sodium. Cell growth, morphological changes and quantification of astaxanthin and chlorophyll were determined by spectrophotometry. Statistical analysis was performed using ANOVA (95%).  Results. Using 0.299 mg/L of sodium acetate a cell growth of 2.0 x 104 Cel/mL and an astaxanthin concentration of 2.530 &#956;g/mL were obtained, while with 1.6 mg/L of sodium acetate the cell growth It was 3.5 x 104 Cel/mL and an astaxanthin concentration of 1.9 &#956;g/mL. The treatment to which 1.6 g L of sodium acetate and 6.4 g/L of sodium chloride were added showed the highest astaxanthin production, 7.3 &#956;g/ml. Treatment with 0.320 g/L sodium acetate + 1.28 g/L sodium chloride showed the highest cell growth with 1.64x105 cells/ml.  Conclusion. This research highlights the importance of initially cultivating the microalgae using the 5-liter Tecferm bioreactor and, after its exponential phase, subjecting it to stress factors with sodium acetate and sodium chloride, thus achieving the highest production of 7.325 &#956;g/ml astaxanthin.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[microalgas]]></kwd>
<kwd lng="es"><![CDATA[crecimiento celular]]></kwd>
<kwd lng="es"><![CDATA[estrés]]></kwd>
<kwd lng="es"><![CDATA[betacaroteno]]></kwd>
<kwd lng="es"><![CDATA[clorofila]]></kwd>
<kwd lng="es"><![CDATA[acetato de sodio]]></kwd>
<kwd lng="en"><![CDATA[microalgae]]></kwd>
<kwd lng="en"><![CDATA[cell growth]]></kwd>
<kwd lng="en"><![CDATA[stress]]></kwd>
<kwd lng="en"><![CDATA[beta-carotene]]></kwd>
<kwd lng="en"><![CDATA[chlorophyll]]></kwd>
<kwd lng="en"><![CDATA[sodium acetate]]></kwd>
</kwd-group>
</article-meta>
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