<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1900-3803</journal-id>
<journal-title><![CDATA[Entramado]]></journal-title>
<abbrev-journal-title><![CDATA[Entramado]]></abbrev-journal-title>
<issn>1900-3803</issn>
<publisher>
<publisher-name><![CDATA[Universidad Libre de Cali]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1900-38032019000100276</article-id>
<article-id pub-id-type="doi">10.18041/1900-3803/entramado.1.5420</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Impacto de la concentración de suero fetal bovino sobre las células epiteliales dentales]]></article-title>
<article-title xml:lang="pt"><![CDATA[Impacto da concentração de soro bovino fetal nas células epiteliais dentárias]]></article-title>
<article-title xml:lang="en"><![CDATA[Impact of bovine fetal serum concentration on dental epithelial cells]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Simancas-Escorcia]]></surname>
<given-names><![CDATA[Victor]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Diaz-Caballero]]></surname>
<given-names><![CDATA[Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad de Cartagena  ]]></institution>
<addr-line><![CDATA[Cartagena ]]></addr-line>
<country>Colombia</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Cartagena  ]]></institution>
<addr-line><![CDATA[Cartagena ]]></addr-line>
<country>Colombia</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2019</year>
</pub-date>
<volume>15</volume>
<numero>1</numero>
<fpage>276</fpage>
<lpage>284</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S1900-38032019000100276&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S1900-38032019000100276&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S1900-38032019000100276&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El esmalte dental, tejido más duro del cuerpo humano, es formado por medio de la diferenciación de células epiteliales conocidas como ameloblastos. Recientemente, las células epiteliales dentales de incisivo de rata han sido utilizadas como modelo celular de eventos fisiopatológicos durante la formación del esmalte dental. Sin embargo, en función de los eventos estudiados es meritorio comprender su comportamiento, particularmente cuando la concentración del Suero Fetal Bovino (SFB) varia. El propósito de este trabajo es evaluar el impacto de la concentración del SFB sobre el crecimiento, proliferación y supervivencia de las células epiteliales dentales. Células epiteliales dentales fueron cultivadas en DMEM/F12, en presencia y ausencia de SFB. Observaciones morfológicas e inmunohistoquímicos de la actina, vimentina y fibronectina fueron realizados. Los resultados permitieron constatar que la ausencia de SFB afectó negativamente la proliferación de las células epiteliales dentales, pero mantuvo una expresión óptima de la actina y vimentina. Se identificó una alteración en la expresión de la fibronectina en las células tratadas en ausencia de SFB. En conclusión, la carencia de SFB disminuyo drásticamente la supervivencia, proliferación y expresión de la fibronectina en las células epiteliales dentales de rata.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Dental enamel, the hardest tissue in the human body is formed by the differentiation of epithelial cells known as ameloblasts. Recently rat incisor dental epithelial cells have been used as a cellular model of pathophysiological events during tooth enamel formation. However depending on the events studied, it is necessary to understand the behavior of these cells, particularly when the concentration of Bovine Fetal Serum (FBS) varies. The purpose of this work is to evaluate the impact of FBS concentration on the growth, proliferation, and survival of dental epithelial cells. Dental epithelial cells were cultured in DMEM/F12 culture medium in the absence and presence of 10% FBS. Morphological and immunohistochemical observations of actin, vimentin, and fibronectin were performed. The results confirm that the absence of FBS negatively affected the proliferation of dental epithelial cells but maintained an optimal expression of actin and vimentin. An alteration in the expression of fibronectin in the cells treated in the absence of FBS was identified. In conclusion, the lack of FBS dramatically decreased the survival, proliferation, and expression of fibronectin in rat dental epithelial cells.]]></p></abstract>
<abstract abstract-type="short" xml:lang="pt"><p><![CDATA[RESUMO O esmalte dentário, o tecido mais duro do corpo humano, é formado pela diferenciação de células epiteliais conhecidas como ameloblastos. Recentemente, células epiteliais dentárias de incisivo de rato têm sido utilizadas como modelo celular de eventos fisiopatológicos durante a formação do esmalte dentário. No entanto, dependendo dos eventos estudados, é meritório entender seu comportamento, particularmente quando a concentração de soro fetal bovino (FBS) varia. O objetivo deste trabalho é avaliar o impacto da concentração de SFB no crescimento, proliferação e sobrevivência de células epiteliais dentárias. Células epiteliais dentárias foram cultivadas em DMEM / F12, na presença e ausência de SFB. Observações morfológicas e imunohistoquímicas de actina, vimentina e fibronectina foram realizadas. Os resultados permitiram confirmar que a ausência de SFB afetou negativamente a proliferação de células epiteliais dentárias, mas manteve uma ótima expressão de actina e vimentina. Uma alteração na expressão de fibronectina nas células tratadas na ausência de SFB foi identificada. Em conclusão, a falta de SFB diminuiu drasticamente a sobrevivência, proliferação e expressão de fibronectina em células epiteliais dentárias de ratos]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Ameloblastos]]></kwd>
<kwd lng="es"><![CDATA[esmalte dental]]></kwd>
<kwd lng="es"><![CDATA[medios de cultivo]]></kwd>
<kwd lng="es"><![CDATA[medio de cultivo libre de suero]]></kwd>
<kwd lng="es"><![CDATA[proliferación celular (Fuente: DeCS)]]></kwd>
<kwd lng="en"><![CDATA[Ameloblasts]]></kwd>
<kwd lng="en"><![CDATA[dental enamel]]></kwd>
<kwd lng="en"><![CDATA[culture media]]></kwd>
<kwd lng="en"><![CDATA[culture media serum-free]]></kwd>
<kwd lng="en"><![CDATA[cell proliferation (Source: Decs)]]></kwd>
<kwd lng="pt"><![CDATA[Ameloblastos]]></kwd>
<kwd lng="pt"><![CDATA[esmalte dentário]]></kwd>
<kwd lng="pt"><![CDATA[meios de cultura]]></kwd>
<kwd lng="pt"><![CDATA[meio de cultura livre de soro]]></kwd>
<kwd lng="pt"><![CDATA[proliferação celular (Fonte: DeCS)]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[BERKOVITZ]]></surname>
<given-names><![CDATA[Barry]]></given-names>
</name>
<name>
<surname><![CDATA[HOLLAND]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[MOXHAM]]></surname>
<given-names><![CDATA[Bernard]]></given-names>
</name>
</person-group>
<source><![CDATA[Oral Anatomy, Histology and Embryology]]></source>
<year></year>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[BORI]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evidence for Bicarbonate Secretion by Ameloblasts in a Novel Cellular Model]]></article-title>
<source><![CDATA[Journal of Dental Research]]></source>
<year>2016</year>
<volume>95</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>588-96</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[BOSKEY]]></surname>
<given-names><![CDATA[Adele]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Bone mineral crystal size]]></article-title>
<source><![CDATA[Osteoporosis International.]]></source>
<year>2003</year>
<volume>14</volume>
<numero>0</numero>
<issue>0</issue>
<page-range>16-21</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[BURNOUF]]></surname>
<given-names><![CDATA[Thierry]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?]]></article-title>
<source><![CDATA[Biomaterials]]></source>
<year>2016</year>
<volume>76</volume>
<page-range>371-87</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[CHIEGO]]></surname>
<given-names><![CDATA[Daniel]]></given-names>
</name>
</person-group>
<source><![CDATA[Essentials of oral histology and embryology: a clinical approach]]></source>
<year>2013</year>
<volume>215</volume>
<edition>4th</edition>
<page-range>55</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[FUJII]]></surname>
<given-names><![CDATA[Sakiko]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Characterization of human dental pulp cells grown in chemically defined serum-free medium]]></article-title>
<source><![CDATA[Biomedical Reports]]></source>
<year>2018</year>
<volume>8</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>350-8</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[GSTRAUNTHALER]]></surname>
<given-names><![CDATA[Gerhard]]></given-names>
</name>
<name>
<surname><![CDATA[SEPPI]]></surname>
<given-names><![CDATA[Thomas]]></given-names>
</name>
<name>
<surname><![CDATA[PFALLER]]></surname>
<given-names><![CDATA[Walter]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Impact of culture conditions, culture media volumes, and glucose content on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic?]]></article-title>
<source><![CDATA[Cellular Physiology and Biochemistry: International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology]]></source>
<year>1999</year>
<volume>9</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>150-72</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[GÜRDAL]]></surname>
<given-names><![CDATA[Özlem]]></given-names>
</name>
<name>
<surname><![CDATA[BAYSAL]]></surname>
<given-names><![CDATA[Kemal]]></given-names>
</name>
<name>
<surname><![CDATA[DURAK]]></surname>
<given-names><![CDATA[Ösmet]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture]]></article-title>
<source><![CDATA[Cytotechnology]]></source>
<year>2018</year>
<volume>70</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>687-700</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[JOCHEMS]]></surname>
<given-names><![CDATA[Carlo]]></given-names>
</name>
<name>
<surname><![CDATA[VAN DER VALK]]></surname>
<given-names><![CDATA[Jan]]></given-names>
</name>
<name>
<surname><![CDATA[STAFLEU]]></surname>
<given-names><![CDATA[Frans]]></given-names>
</name>
<name>
<surname><![CDATA[BAUMANS]]></surname>
<given-names><![CDATA[Vera]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The use of fetal bovine serum: ethical or scientific problem?]]></article-title>
<source><![CDATA[Alternatives to laboratory animals: ATLA]]></source>
<year>2002</year>
<volume>30</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>219-27</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[KAWANO]]></surname>
<given-names><![CDATA[Shintaro]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Establishment of dental epithelial cell line (HAT-7) and the cell differentiation dependent on Notch signaling pathway]]></article-title>
<source><![CDATA[Connective Tissue Research]]></source>
<year>2002</year>
<volume>43</volume>
<numero>2-3</numero>
<issue>2-3</issue>
<page-range>409-12</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[LEE]]></surname>
<given-names><![CDATA[James]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biological alchemy: engineering bone and fat from fat-derived stem cells.]]></article-title>
<source><![CDATA[Annals of Plastic Surgery]]></source>
<year>2003</year>
<volume>50</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>610-7</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[LEI]]></surname>
<given-names><![CDATA[Shuang]]></given-names>
</name>
<name>
<surname><![CDATA[ZHANG]]></surname>
<given-names><![CDATA[Ying]]></given-names>
</name>
<name>
<surname><![CDATA[ZHANG]]></surname>
<given-names><![CDATA[Kaiqiang]]></given-names>
</name>
<name>
<surname><![CDATA[LI]]></surname>
<given-names><![CDATA[Jian]]></given-names>
</name>
<name>
<surname><![CDATA[LIU]]></surname>
<given-names><![CDATA[Lu]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effects of Fluoride on the Expression of Beclin1 and mTOR in Ameloblasts]]></article-title>
<source><![CDATA[Cells Tissues Organs.]]></source>
<year>2015</year>
<volume>200</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>405-12</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MUCCI]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Effect of estrous cow serum during bovine embryo culture on blastocyst development and cryotolerance after slow freezing or vitrification.]]></article-title>
<source><![CDATA[Theriogenology]]></source>
<year>2006</year>
<volume>65</volume>
<numero>8</numero>
<issue>8</issue>
<page-range>1551-62</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[NISHIKAWA]]></surname>
<given-names><![CDATA[Masaki]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[An optimal serum-free defined condition for in vitro culture of kidney organoids]]></article-title>
<source><![CDATA[Biochemical and Biophysical Research Communications]]></source>
<year>2018</year>
<volume>501</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>996-1002</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ROOZAFZOON]]></surname>
<given-names><![CDATA[Reza]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network]]></article-title>
<source><![CDATA[Biochemical and Biophysical Research Communications.]]></source>
<year>2015</year>
<volume>457</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>154-160.</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ROTHOVÁ]]></surname>
<given-names><![CDATA[Michaela]]></given-names>
</name>
<name>
<surname><![CDATA[FENG]]></surname>
<given-names><![CDATA[Jifan]]></given-names>
</name>
<name>
<surname><![CDATA[SHARPE]]></surname>
<given-names><![CDATA[Paul]]></given-names>
</name>
<name>
<surname><![CDATA[PETERKOVÁ]]></surname>
<given-names><![CDATA[Renata]]></given-names>
</name>
<name>
<surname><![CDATA[TUCKER]]></surname>
<given-names><![CDATA[Abigail]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Contribution of mesoderm to the developing dental papilla.]]></article-title>
<source><![CDATA[The International Journal of Developmental Biology]]></source>
<year>2011</year>
<volume>55</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>59-64</page-range></nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[THESLEFF]]></surname>
<given-names><![CDATA[Irma]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Epithelial-mesenchymal signalling regulating tooth morphogenesis]]></article-title>
<source><![CDATA[Journal of Cell Science]]></source>
<year>2003</year>
<volume>116</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>1647-8</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[VAN DER VALK]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.]]></article-title>
<source><![CDATA[Toxicology in vitro: an international journal published in association with BIBRA]]></source>
<year>2010</year>
<volume>24</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>1053-63</page-range></nlm-citation>
</ref>
<ref id="B19">
<label>19</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[WOLFENSON]]></surname>
<given-names><![CDATA[Haguy.]]></given-names>
</name>
<name>
<surname><![CDATA[LAVELIN]]></surname>
<given-names><![CDATA[Irena.]]></given-names>
</name>
<name>
<surname><![CDATA[GEIGER]]></surname>
<given-names><![CDATA[Benjamin]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Dynamic regulation of the structure and functions of integrin adhesions]]></article-title>
<source><![CDATA[Developmental Cell]]></source>
<year>2013</year>
<volume>24</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>447-58</page-range></nlm-citation>
</ref>
<ref id="B20">
<label>20</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[YUE]]></surname>
<given-names><![CDATA[Beatrice]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biology of the Extracellular Matrix: An Overview]]></article-title>
<source><![CDATA[Journal of glaucoma.]]></source>
<year>2014</year>
<page-range>S20-3</page-range></nlm-citation>
</ref>
<ref id="B21">
<label>21</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ZHENG]]></surname>
<given-names><![CDATA[Li]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The tick tock of odontogenesis]]></article-title>
<source><![CDATA[Experimental Cell Research]]></source>
<year>2014</year>
<volume>325</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>83-9</page-range></nlm-citation>
</ref>
<ref id="B22">
<label>22</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ZHENG]]></surname>
<given-names><![CDATA[Li]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Circadian rhythms regulate amelogenesis.]]></article-title>
<source><![CDATA[Bone]]></source>
<year>2013</year>
<volume>55</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>158-65</page-range></nlm-citation>
</ref>
<ref id="B23">
<label>23</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[ZOLLINGER]]></surname>
<given-names><![CDATA[Alicia]]></given-names>
</name>
<name>
<surname><![CDATA[SMITH]]></surname>
<given-names><![CDATA[Michael.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fibronectin, the extracellular glue.]]></article-title>
<source><![CDATA[Matrix Biology: Journal of the International Society for Matrix Biology]]></source>
<year>2017</year>
<volume>60-61</volume>
<page-range>27-37</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
