<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2346-3775</journal-id>
<journal-title><![CDATA[Tropical Grasslands-Forrajes Tropicales]]></journal-title>
<abbrev-journal-title><![CDATA[Trop. Grassl.-Forrajes Trop.]]></abbrev-journal-title>
<issn>2346-3775</issn>
<publisher>
<publisher-name><![CDATA[Centro Internacional de Agricultura Tropical (CIAT)]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2346-37752019000200087</article-id>
<article-id pub-id-type="doi">10.17138/tgft(7)87-95</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Vegetative and micropropagation of leucaena]]></article-title>
<article-title xml:lang="es"><![CDATA[Propagación vegetativa y micropropagación de leucaena]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[IDOL]]></surname>
<given-names><![CDATA[TRAVIS]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[YOUKHANA]]></surname>
<given-names><![CDATA[ADEL]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[SANTIAGO]]></surname>
<given-names><![CDATA[RENIER PAUL]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,University of Hawaii Department of Natural Resources and Environmental Management ]]></institution>
<addr-line><![CDATA[Honolulu HI]]></addr-line>
<country>USA</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>08</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>08</month>
<year>2019</year>
</pub-date>
<volume>7</volume>
<numero>2</numero>
<fpage>87</fpage>
<lpage>95</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S2346-37752019000200087&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S2346-37752019000200087&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S2346-37752019000200087&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract To effectively utilize sterile hybrids of leucaena, efficient protocols for vegetative propagation are needed that meet different user requirements and capabilities. We developed and compared methods for propagating several sterile hybrids of leucaena and compared them with each other and with propagation via seeds for variety K636. Methods included air-layers, rooted cuttings, grafting and tissue culture (micropropagation). All methods required 14&#8210;20 weeks from generation of new shoots on the stock plant to production of rooted plantlets ready to outplant as compared with 6&#8210;8 weeks for seedlings of K636. Successful rooting was highest for air-layers and rooted cuttings. Grafting had lower success owing to a higher skill requirement for the propagator. Tissue culture showed promise, but use of field-grown material was limited by microbial contamination of propagation media. Rooted cuttings are the best option presently for operational-scale propagation, but the method requires a mist system or a carefully controlled non-mist environment. If an effective method can be developed, grafting of young shoots onto a seedling rootstock is an alternative that retains the advantages of a seedling tap root and requires fewer resources than rooted cuttings or tissue culture. In the case of grafting consequences of eventual resprouting of the rootstock deserve attention.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Para poder utilizar los híbridos estériles de leucaena en forma efectiva, se necesitan protocolos eficientes para la propagación vegetativa que se ajusten a las diferentes necesidades y capacidades de los usuarios. En este estudio desarrollamos y comparamos métodos para propagar varios híbridos estériles de leucaena y los comparamos entre sí y con la propagación a través de semillas de la variedad K636. Los métodos incluyeron acodo aéreo, enraizamiento de estacas, injertos y cultivo de tejidos (micropropagación). Todos los métodos requirieron entre 14 y 20 semanas, desde la generación de nuevos brotes de las plantas madre hasta la producción de plántulas enraizadas listas para ser trasplantadas, en comparación con 6&#8210;8 semanas para las plántulas de K636 provenientes de semillas. El enraizamiento más exitoso fue mediante acodo y enraizamiento de estacas. El injerto fue menos exitoso y requirió de una mayor especialización por parte del propagador. El método de cultivo de tejido demostró ser promisorio, pero la contaminación de los medios de propagación por microbios resultó ser una limitante. La técnica de esquejes enraizados es actualmente la mejor opción para la propagación a escala operativa, pero requiere de un cuidadoso control del ambiente, por ejemplo mediante un sistema de humidificación. Si se logra desarrollar una técnica efectiva, el injerto de brotes jóvenes usando como pie de injerto plántulas que provienen de semilla, es una alternativa que mantiene las ventajas de la raíz pivotante de la plántula y requiere menos recursos que el enraizamiento de estacas o el cultivo de tejido. En el caso de injertos las consecuencias de eventuales rebrotes desde las plantas madre merecen atención.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Grafting]]></kwd>
<kwd lng="en"><![CDATA[rooted cuttings]]></kwd>
<kwd lng="en"><![CDATA[sterile hybrids]]></kwd>
<kwd lng="en"><![CDATA[tissue culture]]></kwd>
<kwd lng="es"><![CDATA[Cultivos de tejido]]></kwd>
<kwd lng="es"><![CDATA[enraizamiento de estacas]]></kwd>
<kwd lng="es"><![CDATA[híbridos estériles]]></kwd>
<kwd lng="es"><![CDATA[injertos]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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