Correspondencia: Laura M. Laverde Trujillo e-mail: lauralaverdet@gmail.com
Artículo recibido: 04/08/2011; Artículo aprobado: 12/12/2011
Summary
Bovine besnoitiosis has recently been classed as a re-emerging disease in Western and Central Europe, due to a recent increase in reported cases and a geographic expansion of the disease in cattle herds in continental Europe. The disease has been present in some parts of France, Spain and Portugal for many years, a fact which may be an indicative of the disease's expansion, and it is important to know about it because it has a lot of similar lessons if compared with diseases we have in Colombia. The infection can cause serious adverse effects, both during the acute and the chronic phases that could compromise animal welfare. Bovine besnoitiosis has two distinct sequential clinical stages, namely the acute anasarca stage, which is mainly associated with the proliferation of endozoites in blood vessels, and the chronic scleroderma stage, which is mainly associated with the formation of cysts. The severity of the disease may vary between mild and severe, with possible deaths in seriously affected animals. Many infected animals remain asymptomatic and the only sign of the disease is the presence of cysts in the sclera conjunctiva and/or the vulvae area in cows.
Key words: bovine besnoitiosis, besnoitia besnoiti, anasarca, scleroderma, hyperkeratosis.
Resumen
La besnoitiosis ha sido recientemente clasificada como una enfermedad que resurge en Europa central y occidental, debido a un reciente aumento de casos reportados y una expansión geográfica de la enfermedad entre hatos ganaderos en Europa continental. Se han visto casos en algunos lugares de Francia, España y Portugal por años, lo cual puede indicar que hay expansión de la enfermedad y es importante saber sobre ello porque hay gran similitud de lecciones si se compara con algunas de las enfermedades que se tienen en Colombia. La infección puede causar serios efectos adversos, tanto sea en condición de aguda o crónica, comprometiendo el bienestar del animal. La besnoitiosis bovina tiene dos etapas clínicas consecutivas: la etapa anasarca, principalmente asociada con la proliferación de endozoítos en los vasos sanguíneos, y la esclerodermia crónica, relacionada con la formación de quistes. La gravedad de la enfermedad puede variar entre leve y severa, con posibles muertes en los animales seriamente afectados. Muchos animales infectados permanecen asintomáticos y los únicos signos de la enfermedad son quistes en la conjuntiva esclerótica y/o en el área vulvar de las vacas.
Palabras clave: Besnoitiosis bovina, Besnoitia besnoiti, anasarca, esclerodermia, hiperqueratosis.
A besnoitiosis foi recentemente classificada como uma doença que resurge em Europa central e ocidental, devido a um recente aumento de casos reportados e uma expansão geográfica da doença entre hatos de gado em Europa continental. Viram-se casos em alguns lugares de Franca, Espanha e Portugal por anos, o qual pode indicar que há expansão da doença e é importante saber sobre isso porque há grande similitude de lições se se compara com algumas das doenças que se têm em Colômbia. A infecção pode causar sérios efeitos adversos, tanto seja em condição de aguda ou crônica, comprometendo o bem-estar do animal. A besnoitiosis bovina tem duas etapas clínicas consecutivas: a etapa anasarca, principalmente associada com a proliferação de endozoítos nos copos sanguíneos, e a esclerodermia crônica, relacionada com a formação de quistos. A gravidade da doença pode variar entre leve e severa, com possíveis mortes nos animais seriamente afetados. Muitos animais infectados permanecem assintomáticos e os únicos signos da doença são quistos na conjuntiva esclerótica e/ou no área vulvar das vacas.
Palavras Importantes: Besnoitiosis bovina, Besnoitia besnoiti, anasarca, esclerodermia, hiperqueratosis.
Introduction
Bovine besnoitiosis (also referred as Elephant Skin disease and bovine anasarca) is a disease in cattle caused by an obligate intracellular protozoan parasite that belongs to the phylum apicomplexa species, called Besnoitia besnoiti1-4 which is transmitted by blood sucking insects, or in a smaller proportion via skin contact with other cattle. It is responsible for significant losses in the cattle industry, due to the high morbidity rate (up to 10%5, 6) in affected farms, including mortality, weight loss, prolonged convalescence, definitive or transient infertility in males, (with atrophy, sclerosis and focal necrosis of the testes7, 8) and a decline of milk production, reduction of slaughter weight and abortions -especially in recently affected areas. The cutaneous lesions brings the rejection of hide leather production5 and systemic manifiestations that cause a severe but usually non-fatal disease9. Most infections are mild or subclinical, characterized by the formation of numerous coetaneous and sub-cutaneous microcysts, scleroderma, hyperkeratosis, and alopecia, lowering the quality of the skins for the leather industry. Male sterility or impaired fertility is a common sequel in breeding bulls, and is one of the most negative aspects of the disease in animals that survive infection10.
Knowledge of the clinical course of bovine besnoitiosis is important in order to identify infected animals as soon as possible and prevent the disease's spreading11.
An etiology and life cycle
Besnoitia besnoiti is a cystforming coccidian protozoan parasite, classified within the Toxo-plasmatinae subfamily, Sarcocystidae family, phlymyn Apicompleza and have nine different species: Besnoitia besnoiti, Besnoitia benetti, Besnoitia jellisoni, Besnoitia wallacei, Besnoitia tarandii, Besnoitia darling, Besnoitia caprae, Besnoitia akadoni and Besnoitia oryctofelis2.
Molecular analyses based on large and small subunits of the nuclear ribosomal DNA have shown that Besnoitia spp is closely related to Toxoplasma gondii and Neospora caninum11, 12 and there is a serological cross-reactivity between these related parasites13. There are besnoitia species infecting ungulates14 and other species infecting rodents, lagomorphs and marsupials15. This result represents the knowledge of life cycles, suggesting an evolutionary split within the genus besnoitia towards two lineages, likely link to the intermediate host. This point requires further investigation. The relationship among species is also unclear, especially between B. besnoiti and B. caprae. No significant differences can be seen in the morphological description of bradizoites12-16 but biological properties of B. caprae are different from B. besnoiti, infection in cattle or rodents occurs with B. besnoiti contrary to B. caprae17.
The biology of B. besnoiti, the cause of bovine besnoitiosis, is poorly understood9. Its definitive host has not been identified, and information on potential intermediate hosts is scarce, but it is known that the intermediate host is the cow and another wild ruminant10. In order to investigate potential definitive and intermediate hosts for European isolates of B. besnoiti, domestic dogs, cats, rabbits, guinea pigs (Cavia porcellus), gerbils (Meriones unguiculatus), common voles (Microtus arvalis) and NMRI-mice were inoculated with B. besnoiti isolated from naturally infected German cattle7.
]]> Domestic dogs and cats could not be shown to be definitive hosts of B. besnoiti, but cats seroconverted after feeding on B. besnoiti tissue cysts indicating that B. besnoiti tachyzoites had invaded the cat's tissues18. Moreover, the cats play an important role in the transmission of other species, such as Besnoitia caprae19. A molecular and serological study indicates that European B. besnoiti isolates may infect cats, rabbits, guinea pigs, gerbils, mice and voles. However, a persistence of the parasite could be demonstrated only in voles18.The transmission of the disease and the life cycle of this parasite are to date not fully understood5, 6. A possible route of transmission might be the mechanical, by biting fies such as Tabanus and Stomoxys calcitrans; by medical devices and a direct animal to animal transmission seems to be likely, and seroconversions occurred throughout the year, reaching their highest number in spring. In addition, many seroconversions were reported in the two months before turn-out and could be associated with a high indoors activity of S. calcitrans during this period20. Once the infection is introduced into a herd it can spread fast, in a way that a large proportion of the herd may seroconvert within two or three years21.
These Toxoplasma-like organisms multiply in endothelial, histiocytic and other cells, and produce characteristic large, thick-walled cysts filled with bradyzoites. The cysts of Besnoitia besnoiti (200 - 600 μm in diameter) may appear as white pinhead-shaped nodules that are found in the conjunctive sclera of the eyes, subcutaneous tissue, fascia and mucous membranes of the respiratory and genital tract of infected animals and remain in the animal's body for years11 .
Epidemiology
Besnoitiosis is widespread in Africa, Asia and in the West and Central Europe10-12. It was first reported in Sub-Saharan Africa22 and Asia23. In Europe, bovine besnoitiosis is enzootic only in three countries: Portugal9, Spain24 and the south of France21. Recent epidemiological data confirm an increased number of cases and a geographic expansion of besnoitiosis in cattle herds. Many recent cases have been described in different European countries, and for the first time in Germany7 and Italy25, which may be an indicative of expansion of the disease. Just recently, there was a success in the first detection of cattle besnoitiosis in Central Spain and the stages of this parasite were clearly described during the chronic phase of the disease in a cow that presented tissue cysts, cyst-stages and some of their molecular biological features26. Many infected animals remained asymptomatic and, therefore, serological tests are essential tools for diagnosis22.
There is no report of Besnoitiosis infection in any animal in South America, but Besnoitia sp. infections were reported in naturally infected rabbits, among which tissue cysts were seen in several tissues of five rabbits from a rabbit breeder in La Plata, Argentina27. The global warming generates climate changes that influence the structure and function of natural ecosystems, including host-parasite interactions, wildlife species and disease emergence28. This is why besnoitisis has been recently classified as a re-emerging disease in Europe by the European Food Safety Authority3-5. Another reason of the increase of bovine besnoitiosis is that the seroprevalence and clinical signs can be also be associated with the increasing age of the animals, suggesting a rapid horizontal transmission of the disease29.
Pathogenesis, clinical signs and lesions
As obligate intracellular parasites, endozoites proliferating in cells of blood vessel walls causes degenerative and necrotic vascular lesions, vasculitis and thrombosis in mainly the medium and smaller veins and capillaries of the dermis, subcutis, nasal mucosa, larynx, trachea and testes23. These lesions, together with a toxic effect, apparently cause an increased permeability of blood vessels manifested as anasarca and subcutaneous edema10.
The animals begin to develop clinical signs after approximately two weeks after the infection. There are however some animals in which the incubation period can be extended to two months1-10, and many infected cattle remain asymptomatic or show scleral-conjunctival cysts only30.
Typical clinical cases of bovine besnoitiosis can appear in two stages: the acute anasarca stage, that is associated with the endozoites proliferation in the blood vessels, and the chronic scleroderma stage, associated with the cyst formation2. The acute stage is associated with the proliferative forms (tachyzoites and endozoites). This is when the tachyzoites invade endothelia of blood vessels and the edema appears as the consequence of the vascular damage; the endozoites proliferate and cause degenerative and necrotic vascular lesions30. This stage is characterized by fever, edema, whimper, general weakness and swelling of the superficial lymph nodes. Other clinical signs in this stage are non-specific, such as depression, loss of weight, subcutaneous edema and a pain in joints during the movements that may progress to lameness3-11.
]]> The chronic phase has its origin in the formation of macroscopically cysts visible in the sclera conjunctiva three weeks after the start of the acute phase, and subsequently appears in the mucous membrane of the vestibule vagina and vulvae region. Visible cysts, which look like grains of sugar, may disappear after some months and can appear in a later phase of the disease10-11. This process is non-reversible and chronic besnoitiosis is characterized by hyper-sclerodermia (also called elephant skin), hyperkeratosis, and alopecia in bulls, atrophy, sclerosis and focal necrosis that cause irreversible lesions in the testis2-5. The typical chronic clinical signs in the cattle are progressive thickening and wrinkling of the skin, and the eventual shedding of epidermis affecting the eyelids and the area of the back; the skin signs are accompanied by poor general conditions of the infected animal31.Death may occur in both phases, and the mortality rate is approximately 10%9-32. The most common postmortem changes found in necropsy are: infammation of the pharynx, larynx and trachea, sand-like granules and cysts in the turbinates and nostrils, sand-like granules in the endothelium of large vessels and dermatitis. Histologically, there are epidermal hyperplasia, marked hyperemia, dermal edema and perivascular accumulations of lymphocytes, plasma cells and large histiocytes that will become hosts to parasites accompany the acute febrile stages33.
Differential diagnosis
In tropical parts of the world like Colombia, Besnoitia besnoiti is responsible for skin problems and infertility in cattle. That is why it is often helpful to approach differential diagnoses with dose diseases that involve skin lesions characterized by painful swellings, alopecia and thickening of the skin, and those that shown infertility in males due to testes inflammation34-36.
This organism is predominantly located in tissue-cysts in the skin, mucosal membranes and sclera conjunctiva. In most cases, the typical clinical signs of the disease are cutaneous lesions such as thickening and folding of the skin, dry seborrhea, and hypotrichia or alopecia33 37. Skin diseases in cattle are relatively uncommon, but skin abnormalities are frequently seen during clinical examination. Some of those diseases are:
Bovine herpes mammillitis: the lesions are superficial (involving only the epidermis) and occur predominantly on the cooler parts of the body such as teats and muzzle. Generalized skin lesions can occur accompanied by a transient fever (1 to 3 days). Resolution of the lesion is rapid and results in focal alopecia, but there is no hide damage38.
Streptotrichosis (Dermatophilus congolensis infection): lesions are superficial (often moist and appear as crusts) scabs or 0.5-to 2-cm diameter accumulations of keratinized material. Lesions are common in the skin of the neck, axillary region, inguinal region and perineum. The presence of the organism can be demonstrated by Giemsa staining38.
Hypoderma bovis infection: The parasitic fly larvae of this parasite have a predilection to migrate to the dorsal skin of the back. They cause a nodule with a small central hole through which the larva exits the body, which results in significant hide damage38.
Photosensitization: Dry, faky, infamed areas are confined to the nonpigmented parts of the skin.
Bovine papular stomatitis (lumpy skin disease LSD): Pox-like lesions occur in the skin of the muzzle, oral cavity, and esophagus. There is no generalized disease.
Insect bites: The trauma from insect bites causes local infammation, edema, and pruritus. Insects seldom bite mucous membranes.
The difference from those skin diseases is that in Besnoitiosis the lesions and thick-walled cysts in the skin are caused by sporozoan parasites, which are transmitted mechanically by certain biting flies, and the histological sections will reveal the parasites. Histopathologically, the skins were thickened by hyperplasia of the epidermis, infammatory cellular exudates, fibroplasia and empty or parasitic cysts. Apart from the Besnoitia cyst, the causes of the abnormal thickening of the skin were not ascertained. It was presumed that the lesions of dermatitis and dermatosis might be associated with any of the various causes such as physical trauma and chronic irritations, tick and mite infestations or bacterial and mycotic infections39, 40.
Also besnoitia cysts and some lesions have been observed in the testes, epididymes and blood vessels of bull, causing infammation of the testicles and sterility in those bulls41 and here in Colombia there are some diseases that cause similar signs such as brucelosis, infectious bovine rhinotracheitis, bovine virus diarrhea, bovine genital campylobacteriosis, Trichomona infection, and other non-infectious diseases, such as tumors and traumas42.
Brucellosis is caused by the bacterium Brucella abortus. The organism has an affinity for certain body tissues such as the udder, uterus, lymph nodes, testicles, and accessory sex glands. B.abortus is a very common cause of orchitis. In the bull the injury results in acute infection and it is difficult to cure. The lesion is a necrosis with a subsequent granuloma formation. Usually only one testicle is affected. However, the bull is sterile because the semen is mixed with the inflammatory exudate, or because the testicle suffers thermal degeneration43. Scrotal swelling occurs quickly, getting hot. This is due to inflammatory changes in the tunica and epididymis44.
Infectious bovine rhinotracheitis (IBR) is an infectious disease caused by bovine herpesviruses type 1 (BHV 1). Depending on the subtype of viruses and animal age, the infection shows itself as pneumonia, conjunctivitis, rhinotracheitis, encephalitis, balanopostitis, and reproduction disorders. IBR is a highly contagious disease of the upper respiratory tract and can lead to serious primary or secondary pneumonia. The clinical signs of the disease are nasal discharge, fever and conjunctivitis. Acute disease in dairy cattle is usually accompanied by a severe and prolonged drop in milk production. Adult cows may also suffer from abortion and reduced fertility. Animals suffering from IBR are highly susceptible to secondary bacterial infections45.
Campylobacteriosis is a major infectious cause of infertility in cattle herds46. The vast majority of problems associated with Campylobacter are linked to venereal infection. This disease is spread by infected bulls during service or through poor instrumentation hygiene during an artificial insemination programme42. A bull acquires the disease by mating with an infected cow. Once infected, a bull remains an asymptomatic carrier of the condition46.
Bovine trichomoniasis is a reproductive disease of cattle which can have significant economic impact to cow-calf operations and other cattle enterprises47. Venereal transmission of the causative organism, Trichomonas foetus, can cause one of the most commonly recognized diseases leading to decreased reproductive efficiency in cattle42. There is no consistent observable sign to help with the diagnosis of the disease. The confirmation of the disease requires a demonstration of the organism in the tissue or, most frequently, in culture47.
And the difference is that in besnoitiosis, one or both testes may become permanently athrophic and indurated. Bulls invariably develop an aspermatogenesis, which is usually permanent on account of the severe testicular lesions which usually develop two weeks after the initial clinical reaction41.
Diagnosis
]]> During the first weeks of infection, acutely affected animals may be difficult to diagnose as clinical signs are non-specific48. Characteristic clinical signs tend to be seen with the chronic stage of the disease, primarily thickening of the skin, sclerodermia and the cysts in the sclera, conjunctiva and vulvar regions, and with the development of tissue cysts1. Skin biopsies to confirm the existence of tissue cysts, examining the sample by trichinelloscopy plates or even histopathology are good methods to confirm the disease49.The technique of a skin biopsy was based on the demonstration of Besnoitia besnoiti bradyzoites (cystic stages), which appeared stumpy, each organism 6.2 μ by 3.1 μm in size, banana-shaped 7.7 μm by 1.5 μm in affected skin smears. A more rapid non-surgical technique, scleral conjunctival scraping, revealed similar bradyzoites, thus enhancing the diagnostic value of conjuctival cysts in more chronic infections50.
However, there are a number of diagnostic test such as cytology, histopathology, serology (ELISA, Western blot) and PCR testing available, and they can be useful tools to detect asymptomatic/ sub-clinical cattle for control purposes49, 51. As a simple and inexpensive technique, the modified agglutination test (B-MAT) represents a valuable tool for the diagnosis and study of the epidemiology of bovine besnoitiosis50.
While identification of clinical cases and their histopathological confirmation is relatively simple to carry out, finding subclinical forms of infection is more difficult. Therefore, a more sensitive test for the identification of the etiological agent may be an appropriate diagnostic tool. For this reason, the ITS1 rDNA-sequence-based conventional and real-time PCR have been developed, they are highly sensitive and specific for the detection of B. besnoiti infection in cattle51.
Control
Concerning the control of this disease there are not many effective drugs or vaccines available, although in South Africa there is a live-attenuated vaccine that has been used, and the cattle could be protected from the clinical form of the disease3. B. besnoiti antigens may help to develop new specific and sensitive serological tests based on individual antigens and to identify possible vaccine candidates47. In Europe, at present, only reliable diagnosis together with herd-management measures are available to avoid that non-infected herds acquire the infection in the trade with infected animals. Serological identification of infected cattle is important because the introduction of these animals into naive herds seems to play a major role in the transmission of the parasite2, 52, 53.
Given that the route of transmission of besnoitioisis is not yet fully known, the early detection of infected cattle and the control of their commercial movement might be crucial to improve our knowledge on the biology and the epidemiology of the disease53, 54.
Conclusions
Typical clinical cases of bovine besnoitiosis appear in two distinct sequential stages: The acute anasarca stage, which is mainly associated with a proliferation of endozoites in blood vessels, and the chronic scleroderma stage, which is mainly associated with cyst formation. Affected animals show cutaneous and systemic manifestations and the disease may lead to considerable economic losses. Although generally associated to tropical and subtropical areas, bovine besnoitiosis is now considered an emergent disease in Europe, due to the increasing number of new cases and its apparent geographical expansion. The severity of the disease may vary between mild and severe, or even the death of seriously affected animals. Many infected animals remain asymptomatic and the only sign of the disease is the presence of cysts in sclera conjunctiva and/or vulvar area in cows. A number of diagnostic tests such as cytology, histopathology, serology and PCR testing are available. There are not any effective drugs or vaccines available in Europe at the present time.
Although this is an exotic disease in Colombia, it should be considered for having presentations in tropical countries where the climatic and environmental conditions are similar. Also, the free trade agreements that Colombia already has with other countries allow the entry of animals and animal products that could be potential carriers of this parasite. Another possibility is that the disease is already established in the country and is simply confused with similar lesions.
]]> Domestic animals are often afflicted with various skin problems, some easy to cure, others more complicated, and even some of them can be highly contagious for the human handlers. The effect of skin problems on animal productivity also varies from mild irritations to rapid death, with all kinds of in between stages affecting the productivity and comfort of the animals. It really pays to pay close attention to the health and comfort of domestic animals.Bibliography
1. CASTILLO, J., MARCÉN, J., ORTEGA, L., ÁLVARES G. La besnoitiosis bovina como una enfermedad emergente en Europa. [En línea]. Zaragoza: Portal Veterinaria Albeitar, 2010. Url disponible en: http://albeitar.portalveterinaria.com/noticia/7978/ART%C3%8DCULOS-RUMIANTES/besnoitiosis-bovina-enfermedad-emergente-europa.html [ Links ]
2. LEIGHTON, F. and GAJADHAR, A. Besnoitia spp. and besnoitiosis. In: Samel, W.M., Pybus, M.J., A.A., K. (Eds.), Parasitic Disease Of Wild Mammals. Ames: Manson Publishing, 2001. p. 468-478. [ Links ]
3. EUROPEAN FOOD SAFETY AUTHORITY. Scientific statement on Bovine besnoitiosis. En: EFSA Journal. Vol. 8, No. 2. 15 p. doi:10.2903/j. efsa.2010.1499. 2010. Available online: www.efsa.europa.eu [ Links ]
4. ROSTAHER, A.; et al. Bovine besnoitiosis in germany. Bovine besnoitiosis in Germany. En: Veterinary Dermatology. 2010. Vol. 21. p. 329-334. [ Links ]
5. GOLLINCK, N.; et al. Bovine Besnoitiosis - An emerging parasitic disease of cattle. In: 26th World buiatrics Congress. 2010. Noviembre 14 -18 Santiago, Chile. [ Links ]
6. MEHLHORN, H.; et al. Another African disease in Central Europa: Besnoitiosis of cattle. I. Light and electron microscopical study. En: Parasitol Res. 2009. Vol. 104. p. 861-868. [ Links ]
7. SCHARES, G.; et al. First in vitro isolation of Besnoitia besnoiti from chronically infected cattle in Germany. En: Vet Parasitol. 2009. Vol. 163, No. 4. 315-322. [ Links ]
8. CORTES, H.; et al. Besnoitiosis in bulls in Portugal. En: Vet. Rec. 2005. Vol. 157. p. 262-264. [ Links ]
9. CORTES, H. C.; et al. Isolation of Besnoitia besnoiti from infected cattle in Portugal. En: Vet Parasitol. 2006. Vol. 141. p. 226-233. [ Links ]
10. JACQUIET, P.; LIÉNARD, E. and FRANC, M. Bovine besnoitiosis: Epidemiological and clinical aspects. En: Vet Parasitol. 2010. Vol. 174. p. 30-36. [ Links ]
11. SCHARR, J. C.; et al. The clinical course of bovine besnoitiosis. In: 26th Wold buiatrics Congress. Noviembre 14 -18. 2010. Santiago, Chile. [ Links ]
12. DUBEY, J. P.; et al. Redescription of Besnoitia tarandi(Protozoa: Apicomplexa) from the reindeer (Rangifer tarandus). En: Int.J. Parasitol. 2004. Vol. 34. p. 1273-1287. [ Links ]
13. SHKAP, V.; et al. Immunological relationship between Neospora caninum and Besnoitia besnoiti. En: Vet. Parasitol. 2002. Vol. 106. p. 35-43. [ Links ]
14. DUBEY, J.P.; et al. Redescription of Besnoitia bennetti (Protozoa: Apicomplexa) from the donkey (Equusasinus). En: Int. J. Parasitol. 2005. p. 35:659-672. [ Links ]
15. DUBEY, J.P.; et al. Besnoitia oryctofelisi n. sp. (Protozoa: apicomplexa) from domestic rabbits. En: Parasitology. 2003. Vol. 126. p. 521-539. [ Links ]
16. ELLIS, J.T.; et al. Molecular phylogeny of Besnoitia and the genetic relationships among Besnoitia of cattle, wildebeest and goats. Protist. 2000. Vol. 151. p. 329-336. [ Links ]
17. NJENGA, J.M.; et al. Preliminary findings from an experimental study of caprine besnoitiosis in Kenya. En: Vet. Res. 1993. Commun 17. p. 203-208. [ Links ]
18. BASSO, W.; et al. Exploring the life cycle of Besnoitia besnoiti-experimental infection of putative definitive and intermediate host species. En: Vet Parasitol. 2011. Vol. 174. p. 10-36. [ Links ]
19. NG'ANG'A, C. J. and KASIGAZI, S. Caprine besnoitiosis: studies on the experimental intermediate hosts and the role of the domestic cat in transmission. En: Vet. Parasitol. 1994. Vol. 52. p. 207-210. [ Links ]
20. LIÉNARD, E.; et al. A longitudinal study of Besnoitia besnoiti infections and seasonal abundance of Stomoxys calcitrans in a dairy cattle farm of southwest France. En: Vet. Parasitol. 2011. Vol. 177. p.20-27. [ Links ]
21. ALZIEU, J. P. Re-emerging cattle besnoitiosis (Besnoitia besnoiti) in france: update in clinical and epidemiological aspects. In: 21st International Conference WAAVP, Gante (Belgium). 2007. p. 222. [ Links ]
22. BIGALKE, R. D. New concepts on the epidemiological features of bovine besnoitiosis as determined by laboratory and feld investigations. En: Onderstepoort J. Vet. Res.1968. Vol. 35. p. 3-137. [ Links ]
23. LEE, H. S.; et al. Studies on bovine besnoitiosis in Korea II. A survey on incidence in the enzootic region. En: Korean J. Parasitol. 1970. Vol. 8. p. 76-80. [ Links ]
24. IRIGOIEN, M.; et al. Immunohistochemical study of the cyst of Besnoitia besnoiti. En: Vet Parasitol. 2000. Vol. 91. p. 1-6. [ Links ]
25. AGOSTI, M.; et al. Segnalazione di un focolaio di Besnoitiosi in bovini da carne importati. En: Praxis. 1994. Vol. 15. p. 5-6. [ Links ]
26. FERNÁNDEZ-GARCÍA, A.; et al. First isolation of Besnoitia besnoiti from a chronically infected cow in Spain. En: J Parasitol. 2009. Vol. 95, No. 2. p. 474-476. [ Links ]
27. VENTURINI, L.; et al. Natural Besnoitia sp. infection in domestic rabbits from Argentina. Vet Parasitol. 2002. Vol. 107. p. 273-278. [ Links ]
28. KUTZ, S.J.; et al. The Arctic as a model for anticipating, preventing, and mitigating climate change impacts on host-parasite interactions. Vet Parasitol. 2009. Vol. 163. p. 217-228. [ Links ]
29. FERNÁNDEZ-GARCÍA, A.; et al. Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain. En: Vet. Rec. 2010. Vol. 166. p. 818-822. [ Links ]
30. FERNÁNDEZ-GARCÍA A.; et al. Pattern of recognition of Besnoitia besnoiti tachyzoite and bradyzoite antigens by naturally infected cattle. En: Vet Parasitol. 2009. Vol. 164, No. (2-4). p. 104-110. [ Links ]
31. BOURDEAU, P. J.; et al. Outbreak of bovine besnoitiosis, Besnoitia besnoiti in the west of France and its diagnosis by immunofuores-cence assay. In: In, Proc. IX E. M. O. P., Valencia, 2004. p. 459-460. [ Links ]
32. GRANT, M. Jubb, Kennedy and Palmer's Pathology of Domestic Animals. Volume 1. USA: Elsevier saunders, 2007. p. 709-710. [ Links ]
33. LANGENMAYER, Martin; et al. Pathomorphological changes in skin of Besnoitia besnoiti infected cattle. 2010. Available online: http://www.kenes.com/buiatrics/cd/pdf/974.pdf [ Links ]
34. IGBOKWE, I. O.; ABBA, Y. and NWAGBARA, N. D. Prevalence of Besnoitia Cysts in Pachydermatous Skin Lesions of Cattle in Northeastern Nigeria. En: Nig. J. Exp. Appl. Biol. 2009. Vol. 10, No. 1. p. 31-36. [ Links ]
35. GLOVER, G. J.; SWENDROWSKI, M. and CAWTHORN, R. J. An epizootic of besnoitiosis in captive caribou (Rangifer tarandus caribou), reindeer (Rangifer tarandus tarandus) and mule deer (Odocoileus hemionus hemionus). En: J. Wildl Dis. 1990. Vol. 26. p. 186-195. [ Links ]
36. LIENARD E.; et al. A longitudinal study of Besnoitia besnoiti infections and seasonal abundance of Stomoxys calcitrans in a dairy cattle farm of southwest France. En: Vet.Parasitol. 2011. Vol. 177, No. 1-2. p. 20-27. [ Links ]
37. GOLLNICK, N. S.; GENTILE, A. and SCHARES, G. Diagnosis of bovine besnoitiosis in a bull born in Italy. En: Vet Rec. 2010. Vol. 166. p. 599. [ Links ]
38. SMITH, Bradford. Medicina interna de grandes animales. Barcelona, España: Elsevier, 2010. p. 1082, 1312, 1318. [ Links ]
39. HARGIS, A. M. and GINN, P. E. The integument. In: Pathologic Basis of Veterinary Disease, 4 ed. The Pathologic Basis of Veterinary Disease (McGavin, M. D. and Zachary J. F., eds. St. Louis, Missouri: Mosby Elsevier, 2007. p. 1107-1261. [ Links ]
40. DUBEY, J. P.; et al. Ultrastructure of Besnoitia besnoiti tissue cysts and bradyzoites. En: Journal of Eukaryotic Microbiology. 2003. Vol. 50. p. 240-244. [ Links ]
41. KUMI-DIAKA, J.; et al. Bovine besnoitiosis and its effect on the male reproductive system. En: Theriogenology. 1981. Vol. 16, No. 5. p. 523-530. [ Links ]
42. DANIEL GIVENS, M. and MARLEY M. S. D. Pathogens that cause infertility of bulls or transmission via semen. En: Theriogenology. 2008. Vol. 70. p. 504-507. [ Links ]
43. CARVALHO NETA, A.; et al. Pathogenesis of bovine brucelosis. En: The Veterinary Journal. 2010. Vol. 184. p. 146-155. [ Links ]
44. FOLEY, G.; ANDERSON, W. and STEINBERG, H. Besnoitiosis of the reproductive tract of a blue Duiker (Cephalophus monticola): En: Vet Parasitol. Vol. 1990. Vol. 36, No. (1-2). p. 157-163. [ Links ]
45. NARDELLI, S.; et al. Dynamics of infection and immunity in a dairy cattle Population undergoing an eradication program for Infectious Bovine Rhinotracheitis (IBR). En: Prev. Vet. Med. 2008. Vol. 85. p. 68-80. [ Links ]
46. JIMENEZ, D.; et al. Factors associated with infection by Campylobacter fetus in beef herds in the Province of Buenos Aires, Argentina. Prev. Vet. Med. 2011. Vol. 101. p.157-162. [ Links ]
47. OWEN RAEA, A.; et al. Epidemiology of Tritrichomonas foetus in beef bull populations in Florida. En: Theriogenology. 2004. Vol. 61. p. 605-618. [ Links ]
48. SANNUSI, A. A simple feld diagnostic smear test for bovine besnoitiosis. En: Vet Parasitol. 1991. Vol. 39, No. (1-2). p. 85-188. [ Links ]
49. SCHARES, G.; et al. Evaluation of a commercial ELISA for the specific detection of antibodies against Besnoitia besnoiti. En: Vet Parasitol. 2011. Vol. 175. p. 52-59. [ Links ]
50. WAAP, H.; et al. A modified agglutination test for the diagnosis of Besnoitia besnoiti infection. En: Vet Parasitol. 2011. Vol. 178. p. 217-222. [ Links ]
51. CORTES, HC.; et al. Application of conventional and real time fluorescent ITS 1 rDNA PCR for detection of Besnoitia besnoiti infections in bovine skin biopsies. En: Vet Parasitol. 2007. Vol. 31. p. 352-356. [ Links ]
52. SCHARES, G.; et al. Comparative evaluation of immunofluorescence antibody and novel immunoblot tests for the specific detection of bovine antibodies against Besnoitia besnoiti tachyzoites and bradyzoites. En: Vet. Parasitol. 2010. Vol. 171. p. 32-40. [ Links ]
53. MUTINELLI, F.; et al. Bovine besnoitiosis in imported cattle in Italy. Vet.Parasitol. 2011. Vol. 178. p. 198. [ Links ]
54. GOLDMAN, M. and PIPANO, E. Serological studies on bovine in Israel. Trop. Anim. health prod. 1983. Vol. 15, No. 1. p. 32-38. [ Links ] ]]>