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Agronomía Colombiana
versión impresa ISSN 0120-9965
Resumen
DAVILA, Lida y HIO, Juan Clímaco. Evaluation of activity biocontroller of Arthrobotrys sp. and Paecilomyces sp. on Meloidogyne javanica under in vitro and greenhouse conditions in chrysanthemum (Drendranthema grandiflora Andernson). Agron. colomb. [online]. 2005, vol.23, n.1, pp.91-101. ISSN 0120-9965.
In vitro and greenhouse tests were carried out for evaluating Arthrobotrys sp. and Paecilomyces sp. biocontrol on Meloidogyne javanica eggs and larvae on chrysanthemum plants. 500 nematode larvae and 500 eggs were collected and placed in sterile water suspensions; they were then treated with 1,3. 106; 1,4. 107 y 6,4. 106; 1,14. 107 conidia/mL Arthrobotrys sp. and Paecilomyces sp. concentrations. Evaluations were made by stereoscope every 24 hours. The effect of fungi on M. javanica was quantified 72 hours after incubation, with a control above 50% on larvae and eggs. A random statistical design was employed for plants in soil and sterile compost in a greenhouse. Treatment was applied as follows: individually and combined together; compost alone and inoculated with each fungus; Furadan®; pathogenic target and absolute target. The following variables were evaluated 45 days after inoculation: fresh root weight, length, number of nodules and ambulatory M. javanica nematode juveniles in 100 g soil. The Duncan test (5%) determined that combined fungi controlled M. javanica, having an average of 18 nodules per root, being less efficient when compared to the individual control (an average of 7 and 8 nodules per root, respectively, 45 days after infection). Compost did not control M. javanica, presenting an average of 50 nodules per root. Furadan® treatment presented an average of 25 nodules per root, presenting significant statistical differences respecting treatment with the nematophagous fungi
Palabras clave : Biological control; compost; nematophagous fungi; nematode.