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Revista Facultad de Odontología Universidad de Antioquia

Print version ISSN 0121-246X


DUQUE-ARISTIZABAL, Juan Camilo; ISAZA-AREIZA, Liza María; TOBON-CALLE, Diego  and  LONDONO, Martha Elena. Antibacterial activity of silver nanoparticles immobilized in zinc oxide-eugenol cement against Enterococcus faecalis: an in vitro study. Rev Fac Odontol Univ Antioq [online]. 2019, vol.30, n.2, pp.154-165. ISSN 0121-246X.


the aim of this study is to evaluate the antimicrobial capacity of silver nanoparticles immobilized in a zinc oxide-eugenol (ZOE) cement against Enterococcus faecalis for potential use in endodontic treatments.


experimental in vitro study, performing synthesis of silver nanoparticles (AgNPs) and UV-visible spectroscopy to confirm presence of AgNPs in the prepared solutions. The ZOE mixture was standardized, producing the integrated AgNPs/ZOE material. Scanning electron microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) were used to characterize the integrated material; a Kirby- Bauer assay was finally run to measure the inhibition halos produced by the compound-microorganism interaction.


the UV-visible spectroscopy showed presence of AgNPs in the created solution; both SEM and FTIR show that the AgNPs are integrated into the ZOE system, not altering their properties when performed under conditions like those found in the mouth. The Kirby-Bauer assay shows that all samples had inhibition halos. The AgNPs in guava extract had statistically significant differences with the halos of the other samples (p < 0.05).


the obtained AgNPs show bactericidal activity against Enterococcus faecalis, as a statistically significant difference was found between the AgNPs suspended in guava extract and the other groups; this will be the starting point for future research.

Keywords : endodontics; sealing; cements; microorganisms; Enterococcus faecalis; Kirby-Bauer; nanotechnology; in-vitro.

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