Abstract

SANCHEZ,, MANUEL-ALEJANDRO; VILLEGAS-ESTRADA,, BERNARDO  and  VALENCIA-JIMENEZ,, ARNUBIO. Evaluation of methods for inoculation and diagnosis of the cucumber mosaic virus (CMV). Rev.Bio.Agro [online]. 2021, vol.19, n.1, pp.92-104. ISSN 1692-3561.  https://doi.org/10.18684/bsaa(19)92-104.

The cucumber mosaic virus (CMV) is a limiting agent in the production and quality of agricultural crops whose effective inoculation and reliable diagnosis are essential to implement suitable and timely management strategies. Two protocols for CMV inoculation (Nicotiana benthamiana and N. tabacum cv. Xanthi) as indicator plants and different virus detection methods were evaluated. Infection of plants with CMV was achieved by mechanical transmission and infiltration. The presence of the virus was confirmed by expression of symptoms, serology (ImmunoStrip®), RT-PCR and by sequencing. The results confirmed the effectiveness of the mechanical transmission. The infected plants showed symptoms associated with the pathogen 15 days after inoculation. The serological test allowed detecting the presence of the pathogen only in N. benthamiana plants, while the RT-PCR test in all evaluated plants. The sequencing results showed high nucleotide identity values with the sequences reported in the NBCI for this virus. Mechanical transmission was the most effective method for CMV inoculation, and molecular analysis techniques were more sensitive and reliable for CMV identification, compared to serological test.

Keywords : ImmunoStrip; Disease; Serology.

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