Abstract

ARBOLEDA, FRANCISCO DE JESÚS; GUZMAN, ÓSCAR ADRIÁN  and  MEJIA, LUÍS FERNANDO. EFFECTS OF CASTOR-OIL PLANT (Ricinus communis LINNEO) KETONE EXTRACTS ON THE BURROWING NEMATODE [Radopholus similis(COBB) THORNE] UNDER IN VITRO CONDITIONS. Luna Azul [online]. 2012, n.35, pp.28-47. ISSN 1909-2474.

Nematicides used for phytoparasite nematodes control are expensive and pollute land and water ecosystems. Because of this, an alternative for their handling was looked for, as it was the evaluation of the effect of ketone extracts (Soxhlet's method) in higuerilla (Ricinus communis) roots, stems, leaves and fruits in three concentrations of 25, 50 and 100%, comparing them to water and chemical (Carbofuran) controls on R. similis in vitro conditions. Two mL of each extract were applied on 50 female R. similis placed on Petri dishes. After 48 h, the extracts nematicide and nemostatic effect under a completely randomized experimental design was evaluated. In the three reading times, the higuerilla fruits, roots and leaves ketone extracts in the 100%, concentration had a nematicidal effect between 73 and 89% without significant statistic differences with the chemical control which had values between 82 and 98%. These treatments presented differences statistically significant with the water control which showed lower values between 0.7 and 12%. Bradford's test allowed to detect the presence of proteins in higuerilla ketone tissues extracts confirming that the ketone fruit extract had a high amount of albumin, possibly caster oil, which influenced the increased mortality of R. similis.

Keywords : Nematodes; concentration; protein.

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